-
Article
Open AccessUridine treatment prevents myocardial injury in rat models of acute ischemia and ischemia/reperfusion by activating the mitochondrial ATP-dependent potassium channel
The effect of uridine on the myocardial ischemic and reperfusion injury was investigated. A possible mechanism of its cardioprotective action was established. Two rat models were used: (1) acute myocardial isc...
-
Article
Effect of hypoxia on mitochondrial enzymes and ultrastructure in the brain cortex of rats with different tolerance to oxygen shortage
The mitochondrial structure and the contents of subunits (NDUFV2, SDHA, Cyt b, COX1) of mitochondrial respiratory complexes I–IV as well as of the hypoxia-inducible factor (HIF-1α) in the brain cortex (BC) of ...
-
Article
Open AccessUridine as a protector against hypoxia-induced lung injury
The effect of the activation of the mitochondrial ATP-dependent potassium channel (mitoKATP) on the ultrastructure of rat lung in acute hypoxic hypoxia (7% of oxygen in nitrogen, exposure 30 min) was studied. It ...
-
Article
Formation of lamellar bodies in rat liver mitochondria in hyperthyroidism
In the present work, ultrastructural changes of rat liver mitochondria in hyperthyroidism were studied. Hyperthyroidism was induced in male Wistar rats by daily administration of 100 μg thyroxin per 100 g body we...
-
Article
Energetic, oxidative and ionic exchange in rat brain and liver mitochondria at experimental audiogenic epilepsy (Krushinsky–Molodkina model)
The role of brain and liver mitochondria at epileptic seizure was studied on Krushinsky-Molodkina (KM) rats which respond to sound with an intensive epileptic seizure (audiogenic epilepsy). We didn't find sign...
-
Article
Functioning of the mitochondrial ATP-dependent potassium channel in rats varying in their resistance to hypoxia. Involvement of the channel in the process of animal’s adaptation to hypoxia
The mechanism of tissue protection from ischemic damage by activation of the mitochondrial ATP-dependent K+ channel (mitoKATP) remains unexplored. In this work, we have measured, using various approaches, the ATP...
-
Article
Palmitic Acid Induces the Opening of a Ca2+-Dependent Pore in the Plasma Membrane of Red Blood Cells: The Possible Role of the Pore in Erythrocyte Lysis
Earlier we found that in the presence of Ca2+ palmitic acid (Pal) increases the nonspecific permeability of artificial (planar and liposomal) membranes and causes permeabilization of the inner mitochondrial membr...
-
Article
Physiological aspects of the mitochondrial cyclosporin A-insensitive palmitate/Ca2+-induced pore: tissue specificity, age profile and dependence on the animal’s adaptation to hypoxia
Earlier we found that being added to rat liver mitochondria, palmitic acid (Pal) plus Ca2+ opened a cyclosporin A-insensitive pore, which remained open for a short time. Apparently, this pore is involved in the P...
-
Article
Mitochondrial Ca2+ cycle mediated by the palmitate-activated cyclosporin a-insensitive pore
Earlier we found that in isolated rat liver mitochondria the reversible opening of the mitochondrial cyclosporin A-insensitive pore induced by low concentrations of palmitic acid (Pal) plus Ca2+ results in the br...
-
Article
Ca2+-Induced Phase Separation in the Membrane of Palmitate-Containing Liposomes and Its Possible Relation to Membrane Permeabilization
A Ca2+-induced phase separation of palmitic acid (PA) in the membrane of azolectin unilamellar liposomes has been demonstrated with the fluorescent membrane probe nonyl acridine orange (NAO). It has been shown th...
-
Article
On the mechanism of palmitic acid-induced apoptosis: the role of a pore induced by palmitic acid and Ca2+ in mitochondria
Palmitic acid (Pal) is known to promote apoptosis (Sparagna G et al (2000) Am J Physiol Heart Circ Physiol 279: H2124–H2132) and its amount in blood and mitochondria increases under some pathological condition...
-
Article
Formation of Palmitic Acid/Ca2+ Complexes in the Mitochondrial Membrane: A Possible Role in the Cyclosporin-Insensitive Permeability Transition
A possible role of palmitic acid/Ca2+ (PA/Ca2+) complexes in the cyclosporin-insensitive permeability transition in mitochondria has been studied. It has been shown that in the presence of Ca2+, PA induces a swel...
-
Article
Palmitic and Stearic Acids Bind Ca2+ with High Affinity and Form Nonspecific Channels in Black-Lipid Membranes. Possible Relation to Ca2+-Activated Mitochondrial Pores
A mitochondrial hydrophobic component that forms Ca2+-induced nonspecific ion channels in black-lipid membranes (Mironova et al., 1997) has been purified and its nature elucidated. It consists of long-chain satur...
-
Article
Reconstitution of the Mitochondrial ATP-Dependent Potassium Channel into Bilayer Lipid Membrane1
Electrical properties and regulation of the mitochondrialATP-dependent potassium channel were studied. The channel protein wassolubilized from the mitochondrial membrane using an ethanol/water mixture.Reconsti...
-
Article
Oscillating Ca2+-Induced Channel Activity Obtained in BLM with a Mitochondrial Membrane Component
Oscillations in ion fluxes and membrane potential may be observed in cells and in mitochondria as well. We obtained Ca2+-induced oscillations in channel activity in black-lipid membranes reconstituted with hydrop...
-
Article
Purification of the channel component of the mitochondrial calcium uniporter and its reconstitution into planar lipid bilayers
The purification of the channel-forming component of the mitochondrial calcium uniporter and its channel properties are described. After ethanol and 50% ethanol-water extraction of mitochondria from beef heart...
-
Article
Inhibition of the mitochondrial calcium uniporter by antibodies against a 40-kDa glycorproteinT
Polyclonal rabbit antibodies against a Ca2+-binding mitochondrial glycoprotein were found to inhibit the uniporter-mediated transport of Ca2+ in mitoplasts prepared from rat liver mitochondria. Spermine, a modula...
-
Article
Isolation and properties of Ca2+-transporting glycoprotein and peptide from beef heart mitochondria
The 40,000-dalton glycoprotein and 2000-dalton peptide inducing selective Ca2+-transport through bilayer lipid membranes were isolated from beef heart homogenate and mitochondria. Micromolar concentrations of the...
