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  1. No Access

    Chapter

    Transgenic Ryegrasses(Loliumspp.)

    Ryegrasses (Lolium spp.), together with the closely related fescues (Festucaspp.), are of significant value in temperate grasslands (Jauhar 1993). The commercially most important ryegrasses are Italian or annual ...

    G. Spangenberg, Z. Y. Wang, X. D. Ye, X. L. Wu, I. Potrykus in Transgenic Crops I (2000)

  2. No Access

    Chapter

    Establishment of and Plant Regeneration from Embryogenic Cell Suspensions and their Protoplasts in Forage Grasses

    An efficient system for green plant regeneration from embryogenic suspensions and corresponding protoplasts in the temperate forage and turf grass species: Festuca arundinacea, F. pratensis, F. rubra, Lolium mult...

    Z. Y. Wang, G. Legris, G. Spangenberg in Gene Transfer to Plants (1995)

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    Chapter

    Polyethylene Glycol-Mediated Direct Gene Transfer to Tobacco Protoplasts and Regeneration of Transgenic Plants

    The protocol detailed below describes the procedure for the transient and stable transformation of leaf protoplasts obtained from axenic shoot cultures of Nicotiana tabacum L. cv. Petit Havana SR1, a widely used ...

    G. Spangenberg, I. Potrykus in Gene Transfer to Plants (1995)

  4. No Access

    Chapter

    Genetic Transformation in Festuca arundinacea Schreb. (Tall Fescue) and Festuca pratensis Huds. (Meadow Fescue)

    Tall fescue (Festuca arundinacea Schreb.) is a wind-pollinated, highly self-infertile polyploid perennial cool-season forage, turf, and conservation grass. It is indigenous to Europe, also naturally occuring on t...

    G. Spangenberg, Z.-Y. Wang, M. P. Vallés… in Plant Protoplasts and Genetic Engineering … (1995)

  5. No Access

    Chapter

    Standard Molecular Techniques for the Analysis of Transgenic Plants

    In the following sections, a series of step-by-step protocols for standard molecular techniques widely used in the characterization of transgenic plants and in gene expression studies with plant cells are comp...

    J. Fütterer, A. Gisel, V. Iglesias, A. Klöti, B. Kost… in Gene Transfer to Plants (1995)

  6. No Access

    Chapter

    Somatic Hybridization in Festuca and Lolium

    Several species in the genus Festuca are commonly grown perennial forage and turf grasses of the temperate geographic region. Particularly tall fescue (F. arundinacea Schreb.) has a wide range of distribution as ...

    T. Takamizo, G. Spangenberg in Somatic Hybridization in Crop Improvement I (1994)

  7. No Access

    Chapter

    Somatic Hybridization by Microfusion of Protoplasts

    Somatic hybridization in plants has classically relied on en masse fusion of protoplasts. In general, large populations of protoplasts isolated from both parental forms are mixed and subjected to fusogenic tre...

    G. Spangenberg in Somatic Hybridization in Crop Improvement I (1994)