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Article
Open AccessGenome-wide analysis of Claviceps paspali: insights into the secretome of the main species causing ergot disease in Paspalum spp
The phytopatogen Claviceps paspali is the causal agent of Ergot disease in Paspalum spp., which includes highly productive forage grasses such as P. dilatatum. This disease impacts dairy and beef production by af...
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Article
Assessment of nutritional characteristics of virus-resistant transgenic white clover (Trifolium repens L.) grown under field and glasshouse conditions
White clover (Trifolium repens L.) is an important pasture legume in temperate areas throughout the world, providing fodder for grazing animals and improving soil fertility via symbiotic nitrogen fixation. Howeve...
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Article
Molecular breeding of transgenic white clover (Trifolium repens L.) with field resistance to Alfalfa mosaic virus through the expression of its coat protein gene
Viral diseases, such as Alfalfa mosaic virus (AMV), cause significant reductions in the productivity and vegetative persistence of white clover plants in the field. Transgenic white clover plants ectopically expr...
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Chapter and Conference Paper
Molecular Breeding for Salt Tolerance, Pre-Harvest Sprouting Resistance and Disease Resistance Using Synthetic Hexaploid Wheats, Genetic Transformation, and Associated Molecular Markers
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Article
Novel genotypes of the subtropical grass Eragrostis curvula for the study of apomixis (diplospory)
The aim of this work was to obtain a series of genetically related lines of Eragrostis curvula with different ploidy levels and reproductive modes, which can be used for the discovery of genes associated to diplo...
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Chapter
Low-pollen-allergen ryegrasses: towards a continent free of hay fever?
The ryegrasses (Lolium spp.) are the major grass species sown for forage and amenity use in temperate areas of the world. Pollen of ryegrass is a widespread source of airborne allergens and is a major cause of ha...
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Article
A microsatellite map of white clover
The white clover (Trifolium repens) nuclear genome (n=2x=16) is an important yet under-characterised genetic environment. We have developed simple sequence repeat (SSR) genetic markers for the white clover genome...
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Chapter and Conference Paper
Gene Discovery and Microarray-Based Transcriptome Analysis in Grass Endophytes
A genomic resource of 9411 expressed sequence tags (ESTs) representing 5038 genes has been established for Neotyphodium and Epichloë endophytes. A cDNA spotted microarray interrogating 4195 Neotyphodium and 920 E...
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Chapter and Conference Paper
Field Evaluation of Transgenic White Clover with AMV Immunity and Development of Elite Transgenic Germplasm
Viral diseases such as alfalfa mosaic virus (AMV) cause significant reductions in dry matter yield and persistency of white clover (Trifolium repens L.). Transgenic white clover plants expressing the AMV coat pro...
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Chapter
Biolistic Transformation of Fescues and Ryegrasses
Worldwide permanent pasture is estimated to cover 70% of agriculturally cultivated area. Ryegrasses (Lolium spp.) together with the closely related fescues (Festuca spp.) are of significant value in temperate gra...
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Article
Expression of a sulphur-rich sunflower albumin gene in transgenic tall fescue (Festuca arundinacea Schreb.) plants
Transgenic tall fescue (Festuca arundinacea Schreb.) plants have been generated that express foreign genes encoding a rumen-stable protein rich in sulphur-containing amino acids. The aim was to improve the prote...
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Article
Altered fructan accumulation in transgenic Lolium multiflorum plants expressing a Bacillus subtilis sacB gene
Ryegrasses, like many C3 plants, accumulate fructan, which plays an important role in assimilate partitioning, as the major non-structural storage carbohydrate. The present study describes the transformation ...
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Chapter and Conference Paper
Molecular Breeding of Forage Legumes for Virus Resistance
Viruses such as alfalfa mosaic alfamovirus (AMV), white clover mosaic potex virus (WCMV) and clover yellow vein potyvirus (CYVV), which are members of the three largest groups of plant viruses, have been found...
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Chapter and Conference Paper
Breeding Forage Plants in the Genome Era
Forage plant breeding has been largely based on phenotypic selection following sexual recombination of natural genetic variation found between and within ecotypes. Advances in plant genetic manipulation over t...
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Chapter
Transgenic Ryegrasses(Loliumspp.)
Ryegrasses (Lolium spp.), together with the closely related fescues (Festucaspp.), are of significant value in temperate grasslands (Jauhar 1993). The commercially most important ryegrasses are Italian or annual ...
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Article
Field performance of cell-suspension-derived Lolium perenne L. regenerants and their progenies
Two sets of plants (Lb and Lc), regenerated from different single-genotype-derived embryogenic suspension cultures of Lolium perenne cv Citadel, were evaluated for agronomic traits in a modified polycross design...
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Article
Transgenic Italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells
Transgenic forage-type Italian ryegrass (Lolium multiflorum Lam.) plants have been obtained by microprojectile bombardment of embryogenic suspension cells using a chimeric hygromycin phosphotransferase (hph) gene...
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Chapter
Low density cultures: microdroplets and single cell nurse cultures
The development of low density cell culture systems is mainly based on two assumptions. Firstly, although plant cell (protoplast) cultures are commonly referred to as relatively homogeneous cell populations th...
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Article
Intergeneric symmetric and asymmetric somatic hybridization in Festuca and Lolium
Intergeneric symmetric and asymmetric somatic hybrids have been obtained by fusion of metabolically inactivated protoplasts from embryogenic suspension cultures of tall fescue (Festuca arundinacea Schreb.) and un...
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Article
Genetic engineering of Indica rice in support of sustained production of affordable and high quality food in develo** countries
Indica-type rice provides the staple food for two billion people in Third World countries. Several problems involved in the stable and sustained production of high quality food cannot be solved by traditional ...