![Loading...](https://link.springer.com/static/c4a417b97a76cc2980e3c25e2271af3129e08bbe/images/pdf-preview/spacer.gif)
-
Article
A microsatellite map of white clover
The white clover (Trifolium repens) nuclear genome (n=2x=16) is an important yet under-characterised genetic environment. We have developed simple sequence repeat (SSR) genetic markers for the white clover genome...
-
Chapter
Transgenic Ryegrasses(Loliumspp.)
Ryegrasses (Lolium spp.), together with the closely related fescues (Festucaspp.), are of significant value in temperate grasslands (Jauhar 1993). The commercially most important ryegrasses are Italian or annual ...
-
Article
Field performance of cell-suspension-derived Lolium perenne L. regenerants and their progenies
Two sets of plants (Lb and Lc), regenerated from different single-genotype-derived embryogenic suspension cultures of Lolium perenne cv Citadel, were evaluated for agronomic traits in a modified polycross design...
-
Chapter
Establishment of and Plant Regeneration from Embryogenic Cell Suspensions and their Protoplasts in Forage Grasses
An efficient system for green plant regeneration from embryogenic suspensions and corresponding protoplasts in the temperate forage and turf grass species: Festuca arundinacea, F. pratensis, F. rubra, Lolium mult...
-
Chapter
Polyethylene Glycol-Mediated Direct Gene Transfer to Tobacco Protoplasts and Regeneration of Transgenic Plants
The protocol detailed below describes the procedure for the transient and stable transformation of leaf protoplasts obtained from axenic shoot cultures of Nicotiana tabacum L. cv. Petit Havana SR1, a widely used ...
-
Chapter
Standard Molecular Techniques for the Analysis of Transgenic Plants
In the following sections, a series of step-by-step protocols for standard molecular techniques widely used in the characterization of transgenic plants and in gene expression studies with plant cells are comp...
-
Article
Asymmetric somatic hybridization between tall fescue (Festuca arundinacea Schreb.) and irradiated Italian ryegrass (Lolium multiflorum Lam.) protoplasts
Intergeneric asymmetric somatic hybrids have been obtained by the fusion of metabolically inactivated protoplasts from embryogenic suspension cultures ofFestuca arundinacea (recipient) and protoplasts from a non-...
-
Article
Molecular and cytogenetic characterization of repetitive DNA sequences from Lolium and Festuca: applications in the analysis of Festulolium hybrids
A set of species-specific repetitive DNA sequences was isolated from Lolium multiflorum and Festuca arundinacea. The degree of their species specificity as well as possible homologies among them were determined b...
-
Article
Organelle transfer by microfusion of defined protoplast-cytoplast pairs
Defined cybridization was performed by one-to-one electrofusion (microfusion) of preselected protoplast-cytoplast pairs of male-fertile, streptomycin-resistant Nicotiana tabacum and cytoplasmic male-sterile, stre...
-
Article
Somatic hybridization by microfusion of defined protoplast pairs in Nicotiana: morphological, genetic, and molecular characterization
Somatic hybrid/cybrid plants were obtained by microfusion of defined protoplast pairs from malefertile, streptomycin-resistant Nicotiana tabacum and cytoplasmic male-sterile (cms), streptomycin-sensitive N. tabac...
-
Article
Transgenic rapeseed plants obtained by the microinjection of DNA into microspore-derived embryoids
A novel method in the field of genetic engineering of higher plants is presented: microinjection into multicellular structures which have a high competence for plant regeneration through embryogenesis. Microsp...
-
Article
Individual selection, culture and manipulation of higher plant cells
Due to the heterogeneity in morphology, physiological and morphogenetical capabilities of higher plant cells in mass culture, the development of methods for individually culturing defined cells seemed to be us...