23 Result(s)
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Chapter and Conference Paper
Molecular Breeding for Salt Tolerance, Pre-Harvest Sprouting Resistance and Disease Resistance Using Synthetic Hexaploid Wheats, Genetic Transformation, and Associated Molecular Markers
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Chapter
Low-pollen-allergen ryegrasses: towards a continent free of hay fever?
The ryegrasses (Lolium spp.) are the major grass species sown for forage and amenity use in temperate areas of the world. Pollen of ryegrass is a widespread source of airborne allergens and is a major cause of ha...
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Article
A microsatellite map of white clover
The white clover (Trifolium repens) nuclear genome (n=2x=16) is an important yet under-characterised genetic environment. We have developed simple sequence repeat (SSR) genetic markers for the white clover genome...
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Chapter and Conference Paper
Gene Discovery and Microarray-Based Transcriptome Analysis in Grass Endophytes
A genomic resource of 9411 expressed sequence tags (ESTs) representing 5038 genes has been established for Neotyphodium and Epichloë endophytes. A cDNA spotted microarray interrogating 4195 Neotyphodium and 920 E...
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Chapter and Conference Paper
Field Evaluation of Transgenic White Clover with AMV Immunity and Development of Elite Transgenic Germplasm
Viral diseases such as alfalfa mosaic virus (AMV) cause significant reductions in dry matter yield and persistency of white clover (Trifolium repens L.). Transgenic white clover plants expressing the AMV coat pro...
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Chapter
Biolistic Transformation of Fescues and Ryegrasses
Worldwide permanent pasture is estimated to cover 70% of agriculturally cultivated area. Ryegrasses (Lolium spp.) together with the closely related fescues (Festuca spp.) are of significant value in temperate gra...
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Chapter and Conference Paper
Molecular Breeding of Forage Legumes for Virus Resistance
Viruses such as alfalfa mosaic alfamovirus (AMV), white clover mosaic potex virus (WCMV) and clover yellow vein potyvirus (CYVV), which are members of the three largest groups of plant viruses, have been found...
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Chapter and Conference Paper
Breeding Forage Plants in the Genome Era
Forage plant breeding has been largely based on phenotypic selection following sexual recombination of natural genetic variation found between and within ecotypes. Advances in plant genetic manipulation over t...
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Chapter
Transgenic Ryegrasses(Loliumspp.)
Ryegrasses (Lolium spp.), together with the closely related fescues (Festucaspp.), are of significant value in temperate grasslands (Jauhar 1993). The commercially most important ryegrasses are Italian or annual ...
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Article
Field performance of cell-suspension-derived Lolium perenne L. regenerants and their progenies
Two sets of plants (Lb and Lc), regenerated from different single-genotype-derived embryogenic suspension cultures of Lolium perenne cv Citadel, were evaluated for agronomic traits in a modified polycross design...
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Chapter
Establishment of and Plant Regeneration from Embryogenic Cell Suspensions and their Protoplasts in Forage Grasses
An efficient system for green plant regeneration from embryogenic suspensions and corresponding protoplasts in the temperate forage and turf grass species: Festuca arundinacea, F. pratensis, F. rubra, Lolium mult...
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Chapter
Polyethylene Glycol-Mediated Direct Gene Transfer to Tobacco Protoplasts and Regeneration of Transgenic Plants
The protocol detailed below describes the procedure for the transient and stable transformation of leaf protoplasts obtained from axenic shoot cultures of Nicotiana tabacum L. cv. Petit Havana SR1, a widely used ...
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Chapter
Genetic Transformation in Festuca arundinacea Schreb. (Tall Fescue) and Festuca pratensis Huds. (Meadow Fescue)
Tall fescue (Festuca arundinacea Schreb.) is a wind-pollinated, highly self-infertile polyploid perennial cool-season forage, turf, and conservation grass. It is indigenous to Europe, also naturally occuring on t...
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Chapter
Standard Molecular Techniques for the Analysis of Transgenic Plants
In the following sections, a series of step-by-step protocols for standard molecular techniques widely used in the characterization of transgenic plants and in gene expression studies with plant cells are comp...
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Article
Asymmetric somatic hybridization between tall fescue (Festuca arundinacea Schreb.) and irradiated Italian ryegrass (Lolium multiflorum Lam.) protoplasts
Intergeneric asymmetric somatic hybrids have been obtained by the fusion of metabolically inactivated protoplasts from embryogenic suspension cultures ofFestuca arundinacea (recipient) and protoplasts from a non-...
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Chapter
Somatic Hybridization in Festuca and Lolium
Several species in the genus Festuca are commonly grown perennial forage and turf grasses of the temperate geographic region. Particularly tall fescue (F. arundinacea Schreb.) has a wide range of distribution as ...
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Chapter
Somatic Hybridization by Microfusion of Protoplasts
Somatic hybridization in plants has classically relied on en masse fusion of protoplasts. In general, large populations of protoplasts isolated from both parental forms are mixed and subjected to fusogenic tre...
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Article
Molecular and cytogenetic characterization of repetitive DNA sequences from Lolium and Festuca: applications in the analysis of Festulolium hybrids
A set of species-specific repetitive DNA sequences was isolated from Lolium multiflorum and Festuca arundinacea. The degree of their species specificity as well as possible homologies among them were determined b...
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Article
CMS system inNicotiana: flower development, patterns of mitochondrial DNA and mitochondrial gene expression
A detailed description of in planta floral ontogeny based on scanning electron microscopy, developmental histology and morphology is presented for three different alloplasmic gene-cytoplasmic male-sterile (CMS)Ni...
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Article
Organelle transfer by microfusion of defined protoplast-cytoplast pairs
Defined cybridization was performed by one-to-one electrofusion (microfusion) of preselected protoplast-cytoplast pairs of male-fertile, streptomycin-resistant Nicotiana tabacum and cytoplasmic male-sterile, stre...
