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  1. No Access

    Article

    A microsatellite map of white clover

    The white clover (Trifolium repens) nuclear genome (n=2x=16) is an important yet under-characterised genetic environment. We have developed simple sequence repeat (SSR) genetic markers for the white clover genome...

    B. Barrett, A. Griffiths, M. Schreiber, N. Ellison… in Theoretical and Applied Genetics (2004)

  2. No Access

    Chapter

    Transgenic Ryegrasses(Loliumspp.)

    Ryegrasses (Lolium spp.), together with the closely related fescues (Festucaspp.), are of significant value in temperate grasslands (Jauhar 1993). The commercially most important ryegrasses are Italian or annual ...

    G. Spangenberg, Z. Y. Wang, X. D. Ye, X. L. Wu, I. Potrykus in Transgenic Crops I (2000)

  3. No Access

    Article

    Field performance of cell-suspension-derived Lolium perenne L. regenerants and their progenies

     Two sets of plants (Lb and Lc), regenerated from different single-genotype-derived embryogenic suspension cultures of Lolium perenne cv Citadel, were evaluated for agronomic traits in a modified polycross design...

    F. J. Stadelmann, B. Boller, G. Spangenberg… in Theoretical and Applied Genetics (1998)

  4. No Access

    Chapter

    Establishment of and Plant Regeneration from Embryogenic Cell Suspensions and their Protoplasts in Forage Grasses

    An efficient system for green plant regeneration from embryogenic suspensions and corresponding protoplasts in the temperate forage and turf grass species: Festuca arundinacea, F. pratensis, F. rubra, Lolium mult...

    Z. Y. Wang, G. Legris, G. Spangenberg in Gene Transfer to Plants (1995)

  5. No Access

    Chapter

    Polyethylene Glycol-Mediated Direct Gene Transfer to Tobacco Protoplasts and Regeneration of Transgenic Plants

    The protocol detailed below describes the procedure for the transient and stable transformation of leaf protoplasts obtained from axenic shoot cultures of Nicotiana tabacum L. cv. Petit Havana SR1, a widely used ...

    G. Spangenberg, I. Potrykus in Gene Transfer to Plants (1995)

  6. No Access

    Chapter

    Standard Molecular Techniques for the Analysis of Transgenic Plants

    In the following sections, a series of step-by-step protocols for standard molecular techniques widely used in the characterization of transgenic plants and in gene expression studies with plant cells are comp...

    J. Fütterer, A. Gisel, V. Iglesias, A. Klöti, B. Kost… in Gene Transfer to Plants (1995)

  7. No Access

    Article

    Asymmetric somatic hybridization between tall fescue (Festuca arundinacea Schreb.) and irradiated Italian ryegrass (Lolium multiflorum Lam.) protoplasts

    Intergeneric asymmetric somatic hybrids have been obtained by the fusion of metabolically inactivated protoplasts from embryogenic suspension cultures ofFestuca arundinacea (recipient) and protoplasts from a non-...

    G. Spangenberg, M. P. Vallés, Z. Y. Wang, P. Montavon… in Theoretical and Applied Genetics (1994)

  8. No Access

    Article

    Molecular and cytogenetic characterization of repetitive DNA sequences from Lolium and Festuca: applications in the analysis of Festulolium hybrids

    A set of species-specific repetitive DNA sequences was isolated from Lolium multiflorum and Festuca arundinacea. The degree of their species specificity as well as possible homologies among them were determined b...

    R. Perez-Vicente, L. Petris, M. Osusky, I. Potrykus… in Theoretical and Applied Genetics (1992)

  9. No Access

    Article

    Organelle transfer by microfusion of defined protoplast-cytoplast pairs

    Defined cybridization was performed by one-to-one electrofusion (microfusion) of preselected protoplast-cytoplast pairs of male-fertile, streptomycin-resistant Nicotiana tabacum and cytoplasmic male-sterile, stre...

    G. Spangenberg, E. Freydl, M. Osusky, J. Nagel… in Theoretical and Applied Genetics (1991)

  10. No Access

    Article

    Somatic hybridization by microfusion of defined protoplast pairs in Nicotiana: morphological, genetic, and molecular characterization

    Somatic hybrid/cybrid plants were obtained by microfusion of defined protoplast pairs from malefertile, streptomycin-resistant Nicotiana tabacum and cytoplasmic male-sterile (cms), streptomycin-sensitive N. tabac...

    G. Spangenberg, M. Osusky, M. M. Oliveira, E. Freydl… in Theoretical and Applied Genetics (1990)

  11. No Access

    Article

    Transgenic rapeseed plants obtained by the microinjection of DNA into microspore-derived embryoids

    A novel method in the field of genetic engineering of higher plants is presented: microinjection into multicellular structures which have a high competence for plant regeneration through embryogenesis. Microsp...

    G. Neuhaus, G. Spangenberg, O. Mittelsten Scheid… in Theoretical and Applied Genetics (1987)

  12. No Access

    Article

    Individual selection, culture and manipulation of higher plant cells

    Due to the heterogeneity in morphology, physiological and morphogenetical capabilities of higher plant cells in mass culture, the development of methods for individually culturing defined cells seemed to be us...

    H. -G. Schweiger, J. Dirk, H. -U. Koop, E. Kranz… in Theoretical and Applied Genetics (1987)