-
Article
Occurrence of sweet refuse at disposal sites: rainwater retention capacity and potential breeding opportunities for Aedes aegypti
Nectar is the staple diet of adult mosquitoes in the wild, but its availability is inconsistent and can be affected by rainfall. In urban centers, Aedes vectors commonly use man-made containers as their major hab...
-
Article
Anti-oviposition activities of used sock media against a dengue vector: prospects of eco-friendly control and solutions to pollution
Yearly, huge amounts of sock refuse are discarded into the environment. Socks contain many molecules, and worn ones, which are rich in smell-causing bacteria, have a strong influence on animals’ behaviors. But...
-
Article
Open AccessCoffee and its waste repel gravid Aedes albopictus females and inhibit the development of their embryos
Dengue is a prevalent arboviral disease and the development of insecticide resistance among its vectors impedes endeavors to control it. Coffee is drunk by millions of people daily worldwide, which is associat...
-
Article
Open AccessRecombinant fusion protein of cholera toxin B subunit with YVAD secreted by Lactobacillus caseiinhibits lipopolysaccharide-induced caspase-1 activation and subsequent IL-1 beta secretion in Caco-2 cells
Lactobacillus species are used as bacterial vectors to deliver functional peptides to the intestine because they are delivered live to the intestine, colonize the mucosal surface, and continue to produce the desi...
-
Article
Open AccessColonized Aedes albopictus and its sexual performance in the wild: implications for SIT technology and containment
Mating is a physiological process of crucial importance underlying the size and maintenance of mosquito populations. In sterile and incompatible insect technologies (SIT and IIT), mating is essential for mass ...
-
Article
Open AccessElevation of dopamine level reduces host-seeking activity in the adult female mosquito Aedes albopictus
Mosquito-borne viruses are transmitted to human hosts via blood-feeding behavior of female mosquitoes. Female mosquitoes seek a host to take blood meals (host-seeking behavior). In order to prevent virus infectio...
-
Article
The effects of simulated rainfall on immature population dynamics of Aedes albopictus and female oviposition
Larvae of Aedes albopictus Skuse typically inhabit natural and artificial containers. Since these larval habitats are replenished by rainfall, Ae. albopictus may experience increased loss of immature stages in ar...
-
Article
Novel virus discovery in field-collected mosquito larvae using an improved system for rapid determination of viral RNA sequences (RDV ver4.0)
In this study, we improved a method for rapid determination of viral RNA sequences (RDV) to overcome the limitations of previous versions. The RDV ver4.0 method can detect RNA sequences with at least 1,000 cop...
-
Article
Cloning, Sequence Analysis, and Expression of the Gene Encoding Sphingomonas paucimobilis FP2001 α-L-Rhamnosidase
The gene (rhaM) encoding the α-L-rhamnosidase of Sphingomonas paucimobilis FP2001 was cloned, sequenced, and expressed in Escherichia coli. The rhaM consisted of 3,354 nucleotides and had a promoter and Shine-Dal...
-
Article
Mechanism of the Photocatalytic Inactivation of Lactobacillus casei Phage PL-1 by Titania Thin Film
The mechanism of the inactivation of Lactobacillus casei phage PL-1 suspended in a phosphate buffer by black-light (BL) -catalytic titanium dioxide (TiO2) thin film was studied. Generation of both superoxide anio...
-
Article
Effects of Some Calcium-Related Agents on the Protoplast Transfection of Lactobacillus casei with Phage PL-1 DNA
To clarify the mechanism of Ca2+involvement in the DNA transfer through cell membrane, we studied the effects of Ca2+-chelator, Ca2+-ionophore, and Ca2+-channel blocker on the protoplast transfection of Lactobac...
-
Article
Involvement of host cell energy in the transfection of Lactobacillus casei protoplasts with phage PL-1 DNA
Transfection of Lactobacillus casei ATCC 27092 protoplasts with phage PL-1 DNA was studied under various conditions. The process of transfection was dependent on the incubation temperature, and the apparent activ...
-
Article
High-frequency transformation ofLactobacillus casei with plasmid pHY300PLK by electroporation
To optimize the conditions for transformation ofLactobacillus casei ATCC 27092 cells with plasmid pHY300PLK, a shuttle vector forEscherichia coli andBacillus subtilis, by electroporation, we investigated the effe...
-
Article
Electron microscope studies on the host cell energy requirement for injection of PL-1 phage DNA intoLactobacillus casei
The process of genome DNA injection, after adsorption, by phage PL-1 intoLactobacillus casei ATCC 27092 was monitored by electron microscopy. The DNA injection depended on the incubation temperature, and the appa...
