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Article
Open AccessReverse metabolomics for the discovery of chemical structures from humans
Determining the structure and phenotypic context of molecules detected in untargeted metabolomics experiments remains challenging. Here we present reverse metabolomics as a discovery strategy, whereby tandem m...
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Article
Open AccessBile salt hydrolase catalyses formation of amine-conjugated bile acids
Bacteria in the gastrointestinal tract produce amino acid bile acid amidates that can affect host-mediated metabolic processes1–6; however, the bacterial gene(s) responsible for their production remain unknown. H...
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Article
Open AccessIndividualized microbiotas dictate the impact of dietary fiber on colitis sensitivity
The observation that the intestinal microbiota is central in the development of IBD suggests that dietary fiber, the microbiota’s primary source of nourishment, could play a central role in these diseases. Ac...
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Article
A vitamin-C-derived DNA modification catalysed by an algal TET homologue
Methylation of cytosine to 5-methylcytosine (5mC) is a prevalent DNA modification found in many organisms. Sequential oxidation of 5mC by ten-eleven translocation (TET) dioxygenases results in a cascade of add...
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Article
Open AccessMetabonomics uncovers a reversible proatherogenic lipid profile during infliximab therapy of inflammatory bowel disease
One-third of inflammatory bowel disease (IBD) patients show no response to infliximab (IFX) induction therapy, and approximately half of patients responding become unresponsive over time. Thus, identification ...
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Article
Open AccessSimultaneous Quantification of Amino Metabolites in Multiple Metabolic Pathways Using Ultra-High Performance Liquid Chromatography with Tandem-mass Spectrometry
Metabolites containing amino groups cover multiple pathways and play important roles in redox homeostasis and biosyntheses of proteins, nucleotides and neurotransmitters. Here, we report a new method for simul...
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Article
Open AccessCorrelations of Fecal Metabonomic and Microbiomic Changes Induced by High-fat Diet in the Pre-Obesity State
Obesity resulting from interactions of genetic and environmental factors becomes a serious public health problem worldwide with alterations of the metabolic phenotypes in multiple biological matrices involving...
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Article
Open AccessReprogramming of Seed Metabolism Facilitates Pre-harvest Sprouting Resistance of Wheat
Pre-harvest sprouting (PHS) is a worldwide problem for wheat production and transgene antisense-thioredoxin-s (anti-trx-s) facilitates outstanding resistance. To understand the molecular details of PHS resistance...
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Article
Open AccessMetabonomics of human fecal extracts characterize ulcerative colitis, Crohn’s disease and healthy individuals
This study employs spectroscopy-based metabolic profiling of fecal extracts from healthy subjects and patients with active or inactive ulcerative colitis (UC) and Crohn’s disease (CD) to substantiate the poten...
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Article
NMR-based metabonomic analyses of the effects of ultrasmall superparamagnetic particles of iron oxide (USPIO) on macrophage metabolism
The metabonomic changes in murine RAW264.7 macrophage-like cell line induced by ultrasmall superparamagnetic particles of iron oxides (USPIO) have been investigated, by analyzing both the cells and culture med...
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Article
Characterization of catabolic meta-nitrophenol nitroreductase from Cupriavidus necator JMP134
Cupriavidus necator JMP134 utilizes meta-nitrophenol (MNP) as a sole source of carbon, nitrogen, and energy. The metabolic reconstruction of MNP degradation performed in silico suggested that the mnp cluster migh...
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The reproductive traits of brown trout (Salmo trutta fario L.) from the Yadong River, Tibet
Some key aspects of the reproductive strategy of the brown trout (Salmo trutta fario L.) in the Yadong River, Tibet, including spawning season, age at sexual maturity, fecundity and egg size, have been studied. T...
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Article
The reproductive traits of brown trout (Salmo trutta fario L.) from the Yadong River, Tibet
Some key aspects of the reproductive strategy of the brown trout (Salmo trutta fario L.) in the Yadong River, Tibet, including spawning season, age at sexual maturity, fecundity and egg size, have been studied. T...
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Article
Metabolomic analysis of the response of growing pigs to dietary l-arginine supplementation
Arginine plays an important role regulating nutrient metabolism, but the underlying mechanisms are largely unknown. This study was conducted to determine the effect of dietary arginine supplementation on the m...
