91 Result(s)

Article

Simultaneous de novo calling and phasing of genetic variants at chromosome-scale using NanoStrand-seq

The successful accomplishment of the first telomere-to-telomere human genome assembly, T2T-CHM13, marked a milestone in achieving completeness of the human reference genome. The upcoming era of genome study wi...

**uzhen Bai, Zonggui Chen, Kexuan Chen, Zixin Wu, Rui Wang, Jun’e Liu… in Cell Discovery (2024)

Article

Unveiling gene regulatory networks during cellular state transitions without linkage across time points

Time-stamped cross-sectional data, which lack linkage across time points, are commonly generated in single-cell transcriptional profiling. Many previous methods for inferring gene regulatory networks (GRNs) dr...

Ruosi Wan, Yuhao Zhang, Yongli Peng, Feng Tian, Ge Gao, Fuchou Tang… in Scientific Reports (2024)

Article

DEAD-box helicase 17 (DDX17) protects cardiac function by promoting mitochondrial homeostasis in heart failure

DEAD-box helicase 17 (DDX17) is a typical member of the DEAD-box family with transcriptional cofactor activity. Although DDX17 is abundantly expressed in the myocardium, its role in heart is not fully understo...

Ming**g Yan, Junpeng Gao, Ming Lan, Que Wang… in Signal Transduction and Targeted Therapy (2024)

Article

Map** crossover events of mouse meiotic recombination by restriction fragment ligation-based Refresh-seq

Single-cell whole-genome sequencing methods have undergone great improvements over the past decade. However, allele dropout, which means the inability to detect both alleles simultaneously in an individual dip...

Yan Wang, Yijun Chen, Junpeng Gao, Haoling **e, Yuqing Guo, **gwei Yang… in Cell Discovery (2024)

Article

Author Correction: In vitro expansion of human sperm through nuclear transfer

**aoyu Merlin Zhang, Keliang Wu, Yuxuan Zheng, Han Zhao, Junpeng Gao… in Cell Research (2023)

Article

Base editing-mediated one-step inactivation of the Dnmt gene family reveals critical roles of DNA methylation during mouse gastrulation

During embryo development, DNA methylation is established by DNMT3A/3B and subsequently maintained by DNMT1. While much research has been done in this field, the functional significance of DNA methylation in e...

Qing Li, Jiansen Lu, **di Yin, Yunjian Chang, Chao Wang, Meng Yan… in Nature Communications (2023)

Article

Characterization of mesenchymal stem cells in human fetal bone marrow by single-cell transcriptomic and functional analysis

Bone marrow mesenchymal stromal/stem cells (MSCs) are a heterogeneous population that can self-renew and generate stroma, cartilage, fat, and bone. Although a significant progress has been made toward recogniz...

** Zhang, Ji Dong, **aoying Fan, Jun Yong… in Signal Transduction and Targeted Therapy (2023)

Article

Publisher Correction: High-throughput and high-sensitivity full-length single-cell RNA-seq analysis on third-generation sequencing platform

Yuhan Liao, Zhenyu Liu, Yu Zhang, ** Lu, Lu Wen, Fuchou Tang in Cell Discovery (2023)

Article

Relationships of tumor differentiation and immune infiltration in gastric cancers revealed by single-cell RNA-seq analyses

Gastric cancers are highly heterogeneous malignant tumors. To reveal the relationship between differentiation status of cancer cells and tumor immune microenvironments in gastric cancer, single-cell RNA-sequen...

**n Zhou, **gwei Yang, Yongqu Lu, Yanpeng Ma… in Cellular and Molecular Life Sciences (2023)

Article

High-throughput and high-sensitivity full-length single-cell RNA-seq analysis on third-generation sequencing platform

Yuhan Liao, Zhenyu Liu, Yu Zhang, ** Lu, Lu Wen, Fuchou Tang in Cell Discovery (2023)

Article

scNanoATAC-seq: a long-read single-cell ATAC sequencing method to detect chromatin accessibility and genetic variants simultaneously within an individual cell

Yuqiong Hu, Zhenhuan Jiang, Kexuan Chen, Zhangxian Zhou, **n Zhou… in Cell Research (2023)

Article

Single-cell transcriptomic profiling reveals the tumor heterogeneity of small-cell lung cancer

Small-cell lung cancer (SCLC) is the most aggressive and lethal subtype of lung cancer, for which, better understandings of its biology are urgently needed. Single-cell sequencing technologies provide an oppor...

Yanhua Tian, Qingqing Li, Zhenlin Yang… in Signal Transduction and Targeted Therapy (2022)

Article

Single-cell profiling reveals molecular basis of malignant phenotypes and tumor microenvironments in small bowel adenocarcinomas

Small bowel adenocarcinomas (SBAs) are rare malignant tumors with a high mortality rate, and their molecular characteristics are still largely unexplored. Here we performed single-cell RNA sequencing for tumor...

**gwei Yang, **n Zhou, Ji Dong, Wendong Wang, Yongqu Lu, Yuan Gao… in Cell Discovery (2022)

Article

Single-cell genomic and transcriptomic landscapes of primary and metastatic colorectal cancer tumors

Colorectal cancer (CRC) ranks as the second-leading cause of cancer-related death worldwide with metastases being the main cause of cancer-related death. Here, we investigated the genomic and transcriptomic al...

Rui Wang, **gyun Li, **n Zhou, Yunuo Mao, Wendong Wang, Shuai Gao… in Genome Medicine (2022)

Article

Molecular profiling of human non-small cell lung cancer by single-cell RNA-seq

Lung cancer, one of the most common malignant tumors, exhibits high inter- and intra-tumor heterogeneity which contributes significantly to treatment resistance and failure. Single-cell RNA sequencing (scRNA-s...

Qingqing Li, Rui Wang, Zhenlin Yang, Wen Li, **gwei Yang, Zhijie Wang… in Genome Medicine (2022)

Article

Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis

Patient-derived organoid culture is a powerful system for studying the molecular mechanisms of cancers, especially colorectal cancer (CRC), one of the most prevalent cancers worldwide. There are two main types...

Rui Wang, Yunuo Mao, Wendong Wang, **n Zhou, Wei Wang, Shuai Gao… in Genome Biology (2022)