11 Result(s)
-
Article
Open AccessStaphylococcus carnosus: from starter culture to protein engineering platform
Since the 1950s, Staphylococcus carnosus is used as a starter culture for sausage fermentation where it contributes to food safety, flavor, and a controlled fermentation process. The long experience with S. carno...
-
Article
Excretion of cytoplasmic proteins in Staphylococcus is most likely not due to cell lysis
The excretion of cytoplasmic proteins (ECP) is a long-known phenomenon in bacteria and eukaryotes. So far, it was not possible to associate either a signal peptide-dependent or a signal peptide-independent pat...
-
Article
Inhibition of staphylococcal biofilm-related gene transcription by rhodomyrtone, a new antibacterial agent
Biofilms are one of the most important staphylococcal virulence factors, which are often associated with antibiotic resistance in the organisms. Our previous studies have demonstrated that rhodomyrtone from Rhodo...
-
Article
Binding of ArsR, the repressor of the Staphylococcus xylosus (pSX267) arsenic resistance operon to a sequence with dyad symmetry within the ars promoter
arsR, the first gene of the Staphylococcus xylosus (pSX267) arsenic/antimonite resistance (rs) operon encodes a negative regulatory protein, ArsR, which mediates inducibility of the resistances by arsenic and an...
-
Article
Genetic and biochemical properties of an extracellular neutral metalloprotease from Staphylococcus hyicus subsp. hyicus
The gene encoding the extracellular neutral metalloprotease ShpI from Staphylococcus hyicus subsp. hyicus was cloned. DNA sequencing revealed an ORF of 1317 nucleotides encoding a 438 amino acid protein with Mr o...
-
Article
Cloning and characterization of the scrA gene encoding the sucrose-specific Enzyme II of the phosphotransferase system from Staphylococcus xylosus
By insertional mutagenesis with the staphylococcal transposon Tn551, mutants of Staphylococcus xylosus were isolated that were unable to utilize sucrose. One of these was found to be deficient in sucrose uptake. ...
-
Article
Organization, promoter analysis and transcriptional regulation of the Staphylococcus xylosus xylose utilization operon
The Staphylococcus xylosus xyl genes were cloned in Staphylococcus carnosus by complementation to xylose utilization. Xylose isomerase assays under inducing (xylose present) and non-inducing (xylose absent) condi...
-
Article
Lipase production of Staphylococcus carnosus in a dialysis fermentor
In a newly constructed one-vessel dialysis fermentor, a strain of Staphylococcus carnosus TM300 carrying the lipase secretion plasmid pLipPS1 was used to investigate exoenzyme and biomass production. The bacteria...
-
Article
The molecular organization of the lysostaphin gene and its sequences repeated in tandem
The gene encoding lysostaphin of Staphylococcus staphylolyticus was cloned in Escherichia coli and its DNA sequence was determined. The complete coding region comprises 1440 base pairs corresponding to a precurso...
-
Article
Studies on lipase directed export of Escherichia coli β-lactamase in Staphylococcus carnosus
The lipase (lip) gene of Staphylococcus hyicus was used to study the expression of the Escherichia coli β-lactamase (bla) gene in S. carnosus. The bla gene, devoid of its promotor and most of the signal sequence,...
-
Article
Protoplast transformation of Staphylococcus carnosus by plasmid DNA
Several coagulase-negative staphylococci were investigated for their ability to undergo polyethylene glycolinduced protoplast-transformation with plasmid DNA. Among this group of bacteria it was found that S. car...
