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Chapter and Conference Paper
The contribution of the analytical ultracentrifuge to the technology and development of lipoprotein research, 1948–1991
Application of the analytic ultracentrifuge to lipoproteins began in 1935 with McFarlane’s studies of normal and pathological serum, by which were unexplained multicomponents and distortions in the region of t...
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Chapter and Conference Paper
The Role of Hepatic Triglyceride Lipase in Primates
The catabolism of very low density lipoproteins (VLDL) and production of low density lipoproteins (LDL) has been postulated to proceed via the actions of the enzyme lipoprotein lipase (LPL) bound to the lumina...
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Article
Intercorrelations among plasma high density lipoprotein, obesity and triglycerides in a normal population
The interrelationships among fatness measures, plasma triglycerides and high density lipoproteins (HDL) were examined in 131 normal adult subjects: 38 men aged 27–46, 40 men aged 47–66, 29 women aged 27–46 and...
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Article
Effects of estrogens and progestins on high density lipoproteins
High density lipoprotein (HDL) levels are known to be higher in women than in men, and to increase with estrogen use. To assess the effects of estrogens on HDL subspecies, analytic ultracentrifuge measurements...
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Article
A comparison of simplified methods for lipoprotein quantification using the analytic ultracentrifuge as a standard
Two simplified methods for quantitative lipoprotein analysis have been calibrated and compared with each other using analytic ultracentrifugation as a standard reference procedure. The first method was the Fri...
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Article
Lipid and lipoprotein measurements in a normal adult American population
From a parent population of 774, a subpopulation of 160 normal adults ages 27–66 was randomly selected, 20 from each decade and sex. A detailed comparison was made by analytic ultracentrifugation and complete ...
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Chapter
Pathways of Lipoprotein Metabolism: Integration of Structure, Function and Metabolism
The plasma lipoproteins are complex structures composed of proteins and lipids and involved with the transport of lipids in the circulation. The various lipoproteins are customarily separated into several grou...
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Article
Elemental C, H and N microanalysis of crushed rock and soil samples
A sensitive and reproducible method for elemental analysis of rock and soil samples has been developed using a modified Hewlett-Packard 185 CHN analyzer. Samples are combusted in an inert He atmosphere (with a...
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Article
Subfractionation of Sf 4–105, Sf 4–20 and high density lipoproteins
Subfractionation of the total low density Sf 4–105, the low density Sf 4–20 and high density plasma (or serum) lipoproteins has been accomplished using a cumulative flotation rate procedure. Fractionation employs...
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Article
Graphic presentation of computer-derived schlieren lipoprotein data
Computer produced graphs of lipoprotein spectra visually present a large amount of information and greatly facilitate error detection. Data derived from schlieren patterns in the analytical ultracentrifuge are...
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Article
Flotation rates, molecular weights and hydrated densities of the low-density lipoproteins
A method involving three computer programs is described for characterizing the major component of the Sf 0–12 low-density lipoprotein class by its Sf rate, hydrated density and molecular weight. All necessary inf...
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Article
Comparison of lipoprotein analysis by agarose gel and paper electrophoresis with analytical ultracentrifugation
A comparison has been made of human serum lipoprotein analysis by agarose gel and paper electrophoresis with a standard method of analytical ultracentrifugation. Samples were obtained from 28 patients with var...
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Article
Ultracentrifugal isolation of serum chylomicron-containing fractions with quantitation by infrared spectrometry and NCH elemental analysis
An ultracentrifugal method for isolating chylomicron-containing fractions from serum by flotation, using either standard Spinco swinging-bucket rotors or a specially fabricated swinging-bucket rotor, is descri...
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Article
The use of the computer in serum lipid and lipoprotein analysis
The usefulness of computers in data evaluation is generally recognized; however, the problem of utilizing a computer in the most intelligent manner deserves careful consideration.
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Article
Serum lipoprotein distribution, flotation rates and protein analysis
Flotation rates of the major Sf 0–12 lowdensity lipoprotein component in human serum may be calculated from ultracentrifuge data utilizing two computer programs. One program calculates a classical moving boundary...
