-
Article
Variation in regulation of aflatoxin biosynthesis among isolates of Aspergillus flavus
Two new phenotypes ofAspergillus flavus which exhibit novel patterns of aflatoxin production have been identified and characterized. In one of the new variants ofA. flavus, aflatoxin is made in the absence of car...
-
Article
Production and characterization of antibodies cross-reactive with major aflatoxins
Antibodies cross-reactive with 4 major aflatoxins were demonstrated three weeks after immunization of rabbits with an immunogen which was prepared by conjugating aflatoxin B3 to bovine serum albumin. Aflatoxin B3
-
Article
Cross-reactivity of antibodies against T-2 with deepoxide T-2 toxin
Two types of antibodies raised against T-2 toxin, namely anti-T-2-HS-BSA and anti-3 -Ac -NEOS-HS -BSA, showed good cross-reactivity with deepoxy T-2 toxin. Our results indicate that the epoxide is not an impor...
-
Article
An indirect enzyme-linked immunosorbent assay for the detection of diacetoxyscirpenol in wheat and corn
An indirect enzyme-linked immunosorbent assay (ELISA) was developed for quantitation of diacetoxyscirpenol (DAS) in wheat flour and corn meal. Diacetoxyscirpenol was first converted to its “b”-carboxymethoxyl ...
-
Chapter
Immunochemical methods for diagnosis of mycotoxicoses
Mycotoxins are small molecular weight secondary fungal metabolites and are not immunogenic. However, mycotoxins can be conjugated to a protein or a polypeptide carrier and subsequently be used for immunization...
-
Article
Kinetics of transformation of aflatoxin B1 into aflatoxin M1 in lactating mouse: An ELISA analysis
A new enzyme-linked immunosorbent assay (ELISA) was used to study the kinetics of transformation of aflatoxin B1 into aflatoxin M1 in lactating mice. Aflatoxin M1 concentration in the milk samples reached a maxim...
-
Article
Isolation of altenuisol and altertoxins I and II, minor mycotoxins elaborated byalternaria
A method for the isolation of milligram quantities of 3 minorAlternaria mycotoxins, altenuisol (AS), altertoxin I (AT-I) and altertoxin II (AT-II) was developed. Crude toxin preparation was first subjected to a p...
-
Article
Enzyme-linked immunosorbent assay for T-2 toxin
An enzyme-linked immunosorbent assay (ELISA) was developed for the rapid quantitation of T-2 toxin, a tricothecene mycotoxin produced by members of the genusFusarium. T-2 toxin was first converted to the T-2 hemi...
-
Article
Production and characterization of antibody against aflatoxin M1
Antibody against aflatoxin M1 was obtained after immunization of rabbits with bovine serum albumin-afla M1 oxime conjugate. The antibody has greatest binding efficiency for afla M1, and was less efficient for afl...
-
Article
Modification of hepatotoxic effects of aflatoxin B1 in rabbits by immunization
Reduction of acute toxic effect of aflatoxin B1 was achieved by immunizing the rabbits with small amounts of bovine serum albumin-aflatoxin B1 conjugate. Rabbits after immunization showed lower mortality, near no...
-
Article
Reduction of aflatoxin B1 with zinc borohydride: An efficient preparation of aflatoxicol
A simple and mild reduction of aflatoxin B1, involving treatment of aflatoxin B1 with ethereal zinc borohydride to give 57–65% yield of diastereomeric aflatoxicols, is described.
-
Article
Synthesis of ochratoxins TA and TC, analogs of ochratoxins A and C
Zwei Ochratoxinanaloge, Ochratoxin TA (OTA) und TC (OTC) wurden aus Ochratoxin-α chemisch synthetisiert indem Phenylalanin und sein Äthylester im Molekül durch Tyrosin und Tyrosinäthylester substituiert wurden. D...
-
Article
Fatty acid composition ofChoanephora curubitarum
Crude lipid extracts were prepared fromChoanephora cucurbitarum which had been grown on a glucose-glutamie acid medium for periods up to 12 days. Methanolysis of the extracts and gas chromatography of the esters ...
