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Article
A limited innate immune response is induced by a replication-defective herpes simplex virus vector following delivery to the murine central nervous system
Herpes simplex virus type 1 (HSV-1)—based vectors readily transduce neurons and have a large payload capacity, making them particularly amenable to gene therapy applications within the central nervous system (...
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Article
Herpes simplex virus type 1 shows multiple interactions with sulfonated compounds at binding, penetration, and cell-to-cell passage
Herpes simplex virus type 1 (HSV-1) uses multicomponent mechanisms for binding, penetration, and cell-to-cell passage. These processes are affected by polysulfonated compounds. In this paper we have addressed ...
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Article
Biochip sensors for the rapid and sensitive detection of viral disease
Recent advances in DNA and protein microarray methodology and the emerging technology of cell-based sensors have massively increased the speed and sensitivity with which we can detect viral infections. The adv...
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Protocol
Design of a Herpes Simplex Virus Type 2 Long Oligonucleotide-Based Microarray
The design and construction of a long (75-mer) oligonucleotide-based DNA microarray for herpes simplex virus type 2 transcripts is described. This array is utilized to generate an analysis of HSV-2 transcript ...
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Chapter
HSV Gene Expression during Latent Infection and Reactivation
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Article
The Downstream Activation Sequence of the Strict Late Herpes Simplex Virus Type 1 UL38 Promoter Interacts With hTAFII70, a Component of TFIID
A class of strict late Herpes Simplex Virus Type 1 (HSV-1) promoters contains a conserved sequence element (termed the downstream activation sequence, DAS) located downstream of the transcription start site. T...
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Article
The Exchange of Cognate TATA Boxes Results in a Corresponding Change in the Strength of Two HSV-1 Early Promoters
Previous analysis of two Herpes Simplex Virus Type-1 (HSV-1) promoters controlling expression of mRNA encoding early genes (UL37 and UL50) showed that the UL50 (dUTPase) promoter is at least 6-fold stronger both ...
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Article
Herpes simplex virus recombination vectors designed to allow insertion of modified promoters into transcriptionally “neutral” segments of the viral genome
The use of recombinant viruses has been essential in investigation of the biology of herpes simplex virus (HSV). In this communication we describe a number of viral recombination vectors that we have generated...
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Chapter
The Herpes Simplex Type 1 Virus Latency Gene
The ability of herpesviruses to establish, maintain, and reactivate from latent infections is a hallmark of the group. Latent phase gene expression is under different control than is herpesvirus lytic phase in...
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Article
Transcriptional activation is not responsible for increased levels of autonomously expressed simian virus 40 T-antigen in herpes simplex virus-infected cells
Herpes simples virus type 1 (HSV-1) superinfection of CV-1 cells weakly trans-activated a plasmid-borne metallothionein 1 (MT-1) promoter, but activated the expression of a marker gene controlled by an authentic ...
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Article
Transcriptional activation with concurrent or nonconcurrent template replication has differential effects on transient expression from herpes simplex virus promoters
We have used two methods to induce template replication in order to assess the effect on expression of marker genes controlled by herpes simplex virus type 1 (HSV-1) promoters. One method used the HSV-1 origin...
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Article
Partial rescue of herpes simplex virus neurovirulence with a 3.2 kb cloned DNA fragment
A herpes simplex virus (HSV) intertypic recombinant (RE6) has been previously shown to be non-neurovirulent following direct intracranial inoculation of mice. An in vitro recombination/in vivo selection strate...
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Article
DNA sequence and RNA transcription through a site of recombination in a non-neurovirulent herpes simplex virus intertypic recombinant
RE6 is a herpes simplex type-1 (HSV-1) x herpex simplex type-2 (HSV-2) intertypic recombinant that cannot replicate in the adult mouse nervous system. In the accompanying report, we have shown that HSV-1 seque...
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Article
The kinetics of expression of individual herpes simplex virus type 1 transcripts
We have used specific single-stranded DNA probes cloned in M13 to analyze the kinetics of expression of 20 individual herpes simplex virus type 1 (HSV-1) transcripts, most of known function. The three α transc...
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Article
A bi-functional reporter plasmid for the simultaneous transient expression assay of two herpes simplex virus promoters
We have constructed a transient expression vector (pCAL) containing two reporter genes, chloramphenicol acetyltransferase (CAT) and β-galactosidase (β-gal) for use in studying herpes simplex virus type 1 (HSV-...
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Chapter
Herpesvirus mRNA
In the following pages, we have outlined our general methods for the precise characterization of herpes simplex virus (HSV) transcripts and the information they encode. We have shown that, despite the appearan...
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Chapter
Individual HSV Transcripts
An increasingly complete picture of the phenomenology of animal vims gene expression during productive infection is at hand. In the case of herpesviruses [particularly herpes simplex virus type 1 (HSV-1)], thi...
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Chapter
Isolation and characterization of HSV-1 mRNA
We have reviewed our methods for the isolation, map**, and characterization of HSV-1 mRNA abundant at the three stages of infection. The complexity of viral mRNA increases with each stage of infection. By us...
