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Chapter
Spermiation: Insights from Studies on the Adjudin Model
Spermatogenesis is comprised of a series of cellular events that lead to the generation of haploid sperm. These events include self-renewal of spermatogonial stem cells (SSC), proliferation of spermatogonia by...
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Chapter
Unraveling the Regulation of Cancer/Testis Antigens in Tumorigenesis Through an Analysis of Normal Germ Cell Development in Rodents
Cancer/testis (CT) antigens are proteins aberrantly overexpressed in various tumorigenic cells, but they can also be normally expressed in the mammalian germline. Most CT antigens are highly immunogenic and kn...
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Protocol
Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay
The blood-testis barrier is a unique ultrastructure in the mammalian testis, located near the basement membrane of the seminiferous tubule that segregates the seminiferous epithelium into the basal and the adl...
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Protocol
Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of Actin-, and Microtubule-Based Cytoskeletons
The blood-testis barrier (BTB) is an important ultrastructure in the testis that supports meiosis and postmeiotic spermatid development since a delay in the establishment of a functional Sertoli cell barrier d...
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Article
Open AccessRescue of PFOS-induced human Sertoli cell injury by overexpressing a p-FAK-Y407E phosphomimetic mutant
PFOS induces Sertoli cell injury using testicular cells isolated from rodent testes, but it remains unknown if PFOS has similar effects in humans. Herein, we maintained human Sertoli cells in a mitotically act...
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Article
Open AccessErratum: Perfluorooctanesulfonate (PFOS)-induced Sertoli cell injury through a disruption of F-actin and microtubule organization is mediated by Akt1/2
A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper.
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Article
Open AccessPerfluorooctanesulfonate (PFOS)-induced Sertoli cell injury through a disruption of F-actin and microtubule organization is mediated by Akt1/2
PFOS (perfluorooctanesulfonate, or perfluorooctane sulfonic acid) is an anthropogenic fluorosurfactant widely used in consumer products. While its use in Europe, Canada and the U.S. has been banned due to its ...
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Article
Open AccessRescue of perfluorooctanesulfonate (PFOS)-mediated Sertoli cell injury by overexpression of gap junction protein connexin 43
Perfluorooctanesulfonate (PFOS) is an environmental toxicant used in develo** countries, including China, as a stain repellent for clothing, carpets and draperies, but it has been banned in the U.S. and Cana...
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Chapter
Cell–Cell Interactions, Cell Polarity, and the Blood–Testis Barrier
The study of polarity and its role in cell–cell interactions, such as spermatid polarity and adhesion in the seminiferous epithelium, and blood–testis barrier (BTB) in the testis during the epithelial cycle of...
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Chapter
Gap Junctions and Blood-Tissue Barriers
Gap junction is a cell-cell communication junction type found in virtually all mammalian epithelia and endothelia and provides the necessary “signals” to coordinate physiological events to maintain the homeost...
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Chapter
c-Src and c-Yes are Two Unlikely Partners of Spermatogenesis and their Roles in Blood-Testis Barrier Dynamics
Src family kinases (SFKs), in particular c-Src and c-Yes, are nonreceptor protein tyrosine kinases that mediate integrin signaling at focal adhesion complex at the cell-extracellular matrix interface to regula...
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Chapter
The Apical Ectoplasmic Specialization-Blood-Testis Barrier Functional Axis is A Novel Target for Male Contraception
The blood-testis barrier (BTB), similar to other blood-tissue barriers, such as the blood-brain barrier and the blood-retinal barrier, is used to protect the corresponding organ from harmful substances (e.g., ...
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Chapter
Polarity Protein Complex Scribble/Lgl/Dlg And Epithelial Cell Barriers
The Scribble polarity complex or module is one of the three polarity modules that regulate cell polarity in multiple epithelia including blood-tissue barriers. This protein complex is composed of Scribble, Let...
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Chapter
Role of P-Glycoprotein at the Blood-Testis Barrier on Adjudin Distribution in the Testis
The blood-testis barrier (BTB) is one of the tightest blood-tissue barriers in mammals including rodents and humans. It is used to sequester meiosis I and II, postmeiotic spermatid development via spermiogenes...
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Chapter
Nonreceptor Protein Kinases c-Src, c-Yes, and FAK Are Biomarkers for Male Contraceptive Research
A major obstacle in male contraceptive research and development is the lack of reliable and sensitive biomarkers to monitor the efficacy and potency of candidate compounds under investigation. Since the use of...
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Article
A peptide derived from laminin-γ3 reversibly impairs spermatogenesis in rats
Cellular events that occur across the seminiferous epithelium in the mammalian testis during spermatogenesis are tightly coordinated by biologically active peptides released from laminin chains. Laminin-γ3 dom...
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Article
The biology of interleukin-1: emerging concepts in the regulation of the actin cytoskeleton and cell junction dynamics
Interleukin (IL)-1 is a proinflammatory cytokine with important roles in innate immunity, as well as in normal tissue homeostasis. Interestingly, recent studies have also shown IL-1 to function in the dynamics...
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Protocol
An In Vitro System to Study Sertoli Cell Blood-Testis Barrier Dynamics
The use of an in vitro system based on primary cultures of Sertoli cells isolated from rat testes has greatly facilitated the study of the blood-testis barrier in recent years. Herein, we summarize the detaile...
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Article
A local autocrine axis in the testes that regulates spermatogenesis
A local autocrine axis exists in the testes that regulates spermatogenesis. In this Review, Cheng and Mruk review findings from the past decade that support the presence and reveal the importance of this axis,...
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Chapter
Cross-Talk between Tight and Anchoring Junctions—Lesson from the Testis
Spermatogenesis takes place in the seminiferous tubules in adult testes such as rats, in which develo** germ cells must traverse the seminiferous epithelium while spermatogonia (2n, diploid) undergo mitotic ...
