46 Result(s)
within
Dirk Inzé
Plant Sciences
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Article
Open AccessMonitoring of drought stress and transpiration rate using proximal thermal and hyperspectral imaging in an indoor automated plant phenoty** platform
Thermography is a popular tool to assess plant water-use behavior, as plant temperature is influenced by transpiration rate, and is commonly used in field experiments to detect plant water deficit. Its applica...
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Article
SIAMESE-RELATED1 imposes differentiation of stomatal lineage ground cells into pavement cells
The leaf epidermis represents a multifunctional tissue consisting of trichomes, pavement cells and stomata, the specialized cellular pores of the leaf. Pavement cells and stomata both originate from regulated ...
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Article
Open AccessIMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction
Copy number determination is one of the first steps in the characterization of transgenic plant lines. The classical approach to this, Southern blotting, is time-consuming, expensive and requires massive amoun...
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Article
Open AccessCorrection to: CIN‑like TCP13 is essential for plant growth regulation under dehydration stress
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Article
Open AccessThe heat is on: a simple method to increase genome editing efficiency in plants
Precision genome mutagenesis using CRISPR/Cas has become the standard method to generate mutant plant lines. Several improvements have been made to increase mutagenesis efficiency, either through vector optimi...
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Article
Open AccessCIN-like TCP13 is essential for plant growth regulation under dehydration stress
A dehydration-inducible Arabidopsis CIN-like TCP gene, TCP13, acts as a key regulator of plant growth in leaves and roots under dehydration stress conditions.
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Article
Open AccessRoot engineering in maize by increasing cytokinin degradation causes enhanced root growth and leaf mineral enrichment
Root-specific expression of a cytokinin-degrading CKX gene in maize roots causes formation of a larger root system leading to higher element content in shoot organs.
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Article
Open AccessDevelopment of a novel and rapid phenotype-based screening method to assess rice seedling growth
Rice (Oryza sativa) is one of the most important model crops in plant research. Despite its considerable advantages, (phenotypic) bioassays for rice are not as well developed as for Arabidopsis thaliana. Here, we...
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Article
A genetics screen highlights emerging roles for CPL3, RST1 and URT1 in RNA metabolism and silencing
Post-transcriptional gene silencing (PTGS) is a major mechanism regulating gene expression in higher eukaryotes. To identify novel players in PTGS, a forward genetics screen was performed on an Arabidopsis thalia...
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Article
The role of HEXOKINASE1 in Arabidopsis leaf growth
Here, we used a hxk1 mutant in the Col-0 background. We demonstrated that HXK1 regulates cell proliferation and expansion early during leaf development, and that HXK1 is involved in sucrose-induced leaf growth st...
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Article
Open AccessAIP1 is a novel Agenet/Tudor domain protein from Arabidopsis that interacts with regulators of DNA replication, transcription and chromatin remodeling
DNA replication and transcription are dynamic processes regulating plant development that are dependent on the chromatin accessibility. Proteins belonging to the Agenet/Tudor domain family are known as histone...
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Article
Open AccessLEAF-E: a tool to analyze grass leaf growth using function fitting
In grasses, leaf growth is often monitored to gain insights in growth processes, biomass accumulation, regrowth after cutting, etc. To study the growth dynamics of the grass leaf, its length is measured at reg...
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Article
Transcriptional coordination between leaf cell differentiation and chloroplast development established by TCP20 and the subgroup Ib bHLH transcription factors
The establishment of the photosynthetic apparatus during chloroplast development creates a high demand for iron as a redox metal. However, iron in too high quantities becomes toxic to the plant, thus plants ha...
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Protocol
A Guide to CORNET for the Construction of Coexpression and Protein–Protein Interaction Networks
To enable easy access and interpretation of heterogenous and scattered data, we have developed a user-friendly tool for data mining and integration in Arabidopsis thaliana, designated CORrelation NETworks (acrony...
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Article
Open AccessTranscriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias(Streptophyta), with emphasis on the role of expansin
Streptophyte green algae share several characteristics of cell growth and cell wall formation with their relatives, the embryophytic land plants. The multilobed cell wall of Micrasterias denticulata that rebuilds...
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Article
AtGRP2, a cold-induced nucleo-cytoplasmic RNA-binding protein, has a role in flower and seed development
The glycine-rich protein AtGRP2 is one of the four members of the cold-shock domain (CSD) protein family in Arabidopsis. It is characterized by the presence of a nucleic acid-binding CSD domain, two glycine-rich ...
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Chapter and Conference Paper
Integrating Transcriptional and Metabolic Profiling to Unravel Secondary Metabolite Biosynthesis in Plants
Plants exhibit an overwhelming metabolic capacity and the quest to discover all secondary metabolites has barely begun. Only a fraction of the 500,000 plant species estimated to exist has been formally describ...
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Article
Lateral Root Initiation or the Birth of a New Meristem
Root branching happens through the formation of new meristems out of a limited number of pericycle cells inside the parent root. As opposed to shoot branching, the study of lateral root formation has been comp...
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Article
The Arabidopsis leaf as a model system for investigating the role of cell cycle regulation in organ growth
The role of cell cycle regulation and cell division in plant growth and organ development is controversial. Some experimental data are most easily interpreted from the ‘cellular perspective’ that cell division...
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Chapter
Improvements of the Molecular Toolbox for Cell Cycle Studies in Tobacco BY-2 Cells
The cell cycle is a highly ordered process consisting of four consecutive phases, G1, S (DNA replication), G2, and M (mitosis) that lead to the formation of two daughter cells. During G1 phase, the cell monito...
