24 Result(s)

Article

A comparative study of the rat heart sarcolemmal Ca²⁺-dependent ATPase and myosin ATPase

In order to gain some information regarding Ca²⁺-dependent ATPase, the enzyme was purified from cardiac sarcolemma and its properties were compared with Ca²⁺-ATPase activity of myosin purified from rat heart. Bot...

Vijayan Elimban, Dayuan Zhao, Naranjan S. Dhalla in Molecular and Cellular Biochemistry (1987)

Article

Plant regeneration from protoplasts of Indica type rice and CMS rice

Cell suspensions were established from 12 rice genotypes including Indica type, Japonica type and cytoplasmic male sterile (cms) rice lines of both types. 133 green plants were regenerated from protoplasts iso...

Dayuan Wang, Paul D. Miller, Maro R. Söndahl in Plant Cell Reports (1989)

Article

Phosphorylation of aortic plasma membranes by protein kinase C

Incubation of plasma membranes isolated from bovine aorta with either 0.5 mM CaCl₂ or with a phorbol ester (1 µM phorbol 12,13-dibutyrate) and phosphatidylserine in an EGTA-containing buffer resulted in the phosp...

Dayuan Zhao, Karen R. Dell, Morley D. Hollenberg… in Molecular and Cellular Biochemistry (1991)

Article

Characterization of the purified rat heart plasma membrane Ca²⁺/Mg²⁺ ATPase

The purified Ca²⁺/Mgu2+ ATPase from rat heart plasma membrane was activated by Ca²⁺ and Mg²⁺ with Ka values of 1.47 mM and 2.51 mM, respectively; other divalent cations also activated the enzyme but to a lesser e...

Dayuan Zhao, Vijayan Elimban, Naranjan S. Dhalla in Molecular and Cellular Biochemistry (1991)

Article

Purification and composition of Ca²⁺/Mg²⁺ ATPase from rat heart plasma membrane

The Ca²⁺/Mg²⁺ ATPase, which is activated by millimolar concentrations of Ca²⁺ or Mg²⁺, was solubilized from rat heart plasma membrane by employing lysophosphatidylcholine, CHAPS, Nal, EDTA and Tris-HCI at pH 7.4....

Dayuan Zhao, Naranjan S. Dhalla in Molecular and Cellular Biochemistry (1991)

Article

Comparison of an endogenous protein kinase C substrate in rat aorta with rat brain MARCKS

We have compared the properties of a rat aorta-derived protein kinase C substrate (p75) with those of 80 kDa kinase C substrates from rat brain (MARCKS) and rabbit aorta (p80). Rat aortic p75 appeared to be cl...

Dayuan Zhao, Morley D. Hollenberg, David L. Severson in Molecular and Cellular Biochemistry (1992)

Article

Subzonal fertilization of mouse round spermatids

Mouse round spermatids were electrofused with homologous mature oocytes to examine the behaviour of their nuclei within the ooplasm and the abilities of development. A single spermatid was injected in the peri...

Ling Liu, Qingyuan Sun, Chongwen Duan, Hui Liu… in Science in China Series C: Life Sciences (1997)

Article

Immunolocation of antisperm monoclonal antibody 6B10 and corresponding antigen

An antisperm monoclonal antibody 6B10 was produced by hybridoma technique of the isotype IgG. The monoclonal antibody was purified by means of ammonium sulfate precipitation and protein A-Sepharose C1-4B affin...

Shaorong Gao, Guojun Hu, Chongwen Duan, Hui Liu… in Science in China Series C: Life Sciences (1999)

Article

Antigen changes of monoclonal antibody MSH27 in process of post-testicular maturation (in mice)

An anti-mouse spermatozoon monoclonal antibody, MSH27, as well as its purified antigen, can block sperm-egg membrane fusion. As a candidate protein for sperm-egg membrane fusion, the sperm antigen was investig...

Dazhong Zhuang, Zhiming Han, **angfen Song… in Science in China Series C: Life Sciences (1999)

Article

Biodiversity conservation in China: Legislation, Plans and Measures

China is one of the “megadiversity” countries with over 30,000 species of higher plants and 6347 species of vertebrates, including numerous endemic species and relict species. The vast territory, with its vari...

Haigen Xu, Shunqing Wang, Dayuan Xue in Biodiversity & Conservation (1999)

Article

The giant panda (Ailuropoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg

The giant panda skeletal muscle cells, uterus epithelial cells and mammary gland cells from an adult individual were cultured and used as nucleus donor for the construction of intenpecies embryos by transferri...

Dayuan Chen, Qingyuan Sun, Jilong Liu… in Science in China Series C: Life Sciences (1999)

Article

Valuing ecological functions of biodiversity in Changbaishan Mountain Biosphere Reserve in Northeast China

Conservation of biodiversity can generate considerable indirect economic value and this is being increasingly recognized in China. For a forest ecosystem type of a nature reserve, the most important of its val...

Dayuan Xue, Clem Tisdell in Biodiversity & Conservation (2001)

Protocol

Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System

BacMams are modified baculoviruses that contain mammalian expression cassettes for gene delivery and expression in mammalian cells. The BacMam system combines the advantages of viral transient expression, ease...

James A. Fornwald, Quinn Lu, Dayuan Wang… in Baculovirus and Insect Cell Expression Pro… (2007)

Article

Identification of three major R genes responsible for broad-spectrum blast resistance in an indica rice accession

An indica rice accession HR4 was found to exhibit good resistance to rice blast in previous research. Inoculation with 116 different M. oryzae isolates revealed that HR4 has the broadest resistance spectrum of th...

Wuming **ao, Qiyun Yang, Dayuan Sun, Hui Wang, Tao Guo, Yongzhu Liu… in Molecular Breeding (2015)

Article

Cultural Uses, Ecosystem Services, and Nutrient Profile of Flowering Quince (Chaenomeles speciosa) in the Highlands of Western Yunnan, China

Lixin Yang, Selena Ahmed, John Richard Stepp, Yanqinag Zhao, Ma Jun Zeng… in Economic Botany (2015)

Chapter

Global Climate Change, Ecological Stress, and Tea Production

Tea is the second most consumed beverage worldwide, and the production of tea is of economic importance in over 50 countries. As a woody perennial, tea plants are cultivated in production systems for numerous ...

Selena Ahmed, Tim Griffin, Sean B. Cash… in Stress Physiology of Tea in the Face of Cl… (2018)