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Protocol
Diabetes in Mice with Monogenic Obesity: The db/db Mouse and Its Use in the Study of Cardiac Consequences
The leptin receptor deficient db/db mouse has served as a rodent model for obesity and type 2 diabetes for more than 40 years. Diabetic features in db/db mice follow an age-dependent progression, with early insul...
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Article
What are the Biochemical Mechanisms Responsible for Enhanced Fatty Acid Utilization by Perfused Hearts from Type 2 Diabetic db/db Mice?
It is generally accepted that diabetic hearts have an altered metabolic phenotype, with enhanced fatty acid (FA) utilization. The over-utilization of FA by diabetic hearts can have deleterious functional conse...
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Chapter
Diabetes and Cardiac Dysfunction
Type 2 diabetes is associated with a marked increase in cardiovascular disease. This review summarizes some of the experimental evidence supporting the existence of a diabetic cardiomyopathy, defined as ventri...
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Chapter
Cardiac Function in Perfused Hearts from Diabetic Mice
Non-insulin dependent (type 2) diabetes mellitus accounts for more than 90% of all cases of diabetes. An increased incidence of cardiovascular diseases is the most common complication of NIDDM.1,2 The cardiac com...
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Article
The isolated working mouse heart: methodological considerations
Our aim was to develop a working isolated murine heart model, as the extensive use of genetically engineered mice in cardiovascular research requires development of new miniaturized technology. Left ventricul...
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Article
Long term incubation of cardiac myocytes with oleic acid and very-low density lipoprotein reduces heparin-releasable lipoprotein lipase activity
An exogenous [3H]triolein emulsion was hydrolyzed by intact cardiac myocytes with functional LPL located on the cell surface. This surface-bound LPL could be released into the medium when cardiac myocytes were in...
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Article
Comparison of an endogenous protein kinase C substrate in rat aorta with rat brain MARCKS
We have compared the properties of a rat aorta-derived protein kinase C substrate (p75) with those of 80 kDa kinase C substrates from rat brain (MARCKS) and rabbit aorta (p80). Rat aortic p75 appeared to be cl...
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Article
Inhibition of myocardial lipoprotein lipase by U-57,908 (RHC 80267)
U-57,908 (RHC 80267) was shown to inhibit lipoprotein lipase (LPL) activity in cardiac myocytes from rat hearts; the concentrations required for inhibition to 50% of control activity were 1.1 μM and 2.5 μM for...
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Chapter
Long term Incubation of Cardiac Myocytes with Oleic Acid and Very-low Density Lipoprotein Reduces Heparin-releasable Lipoprotein Lipase Activity
An exogenous [3H]triolein emulsion was hydrolyzed by intact cardiac myocytes with functional LPL located on the cell surface. This surface-bound LPL could be released into the medium when cardiac myocytes were in...
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Article
Phosphorylation of aortic plasma membranes by protein kinase C
Incubation of plasma membranes isolated from bovine aorta with either 0.5 mM CaCl2 or with a phorbol ester (1 µM phorbol 12,13-dibutyrate) and phosphatidylserine in an EGTA-containing buffer resulted in the phosp...
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Article
Protein kinase C does not regulate diacylglycerol metabolism in aortic smooth muscle cells
The effect of a reduction in protein kinase C activity on the metabolism of exogenous [3H]diC8 by freshly isolated smooth muscle cells from rabbit aorta and cultured A10 smooth muscle cells was determined. The me...
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Article
Effects of hypoxia on lipolysis in isolated rat myocardial cells
The effect of hypoxia on myocardial lipolysis (glycerol release) was investigated in freshly isolated, calcium-tolerant rat ventricular myocytes. Hypoxia was produced by gassing the incubation medium (Joklik-m...
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Article
Effect of taxol on the heparin-induced secretion of lipoprotein lipase from cardiac myocytes
The heparin-induced secretion of LPL into the incubation medium of cardiac myocytes occurred in two phases: a rapid release (5–10 min), followed by a slower rate of release (10–60 min). Reducing the incubation...
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Chapter
Effects of hypoxia on lipolysis in isolated rat myocardial cells
The effect of hypoxia on myocardial lipolysis (glycerol release) was investigated in freshly isolated, calcium-tolerant rat ventricular myocytes. Hypoxia was produced by gassing the incubation medium (Joklik-m...
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Chapter
Effect of taxol on the heparin-induced secretion of lipoprotein lipase from cardiac myocytes
The heparin-induced secretion of LPL into the incubation medium of cardiac myocytes occurred in two phases: a rapid release (5–10 min), followed by a slower rate of release (10–60 min). Reducing the incubation...
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Article
Secretion of lipoprotein lipase from myocardial cells isolated from adult rat hearts
Heparin (5 U/ml) induced the release of LPL into the incubation medium of cardiac myocytes isolated from adult rat hearts. The secretion of LPL occurred in two phases: a rapid release (5–10 min of incubation w...
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Article
Properties of monoacylglycerol lipase in rabbit aorta
Monoacylglycerol lipase activity was characterized in a soluble preparation from rabbit aorta (intima-media) obtained by combining a 100,000×g supernatant fraction with activity solubilized from the 100,000×g ...
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Article
Stimulation of a neutral triacylglycerol hydrolase from rat heart by phosphatidylethanolamine and lysophosphatidylethanolamine
Triacylglycerol hydrolase activity measured at pH 7.5 in a pH 5.2 precipitate fraction from rat heart was increased two-to three-fold by the presence of phosphatidylethanolamine (PE) or lysophosphatidylethanol...
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Article
Inhibition of the hormone-sensitive lipase in adipose tissue by long-chain fatty acyl coenzyme A
The effects of free fatty acids and fatty acyl esters of coenzyme A and carnitine on the activity of a hormone-sensitive lipase preparation made from pigeon adipose tissue were determined. Oleic acid (100 μM) ...
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Chapter
Divalent Metal Ions and the Regulation of Pyruvate Dehydrogenase
Pyruvate dehydrogenase catalyzes the irreversible decarboxylation of pyruvate to form acetyl-CoA. The enzyme is an exclusively mitochondrial multienzyme complex with a molecular weight approaching 10 million. ...
