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Open AccessDetection of pathogenic Escherichia coli on potentially contaminated beef carcasses using cassette PCR and conventional PCR
Over a one year period, swabs of 820 beef carcasses were tested for the presence of Shiga toxin-producing Escherichia coli by performing Polymerase Chain Reaction (PCR) in a novel technology termed “cassette PCR”...
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Article
Open AccessApplication of lab-on-a-chip multiplex cassette PCR for the detection of enterohemorrhagic Escherichia coli
Fast molecular detection methods benefit from ready-to-run lab-on-a-chip molecular assays with minimum preparation time. Detection efficiency of such methods can improve if multiple targets are detected simult...
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Article
Open AccessA lab-on-chip for malaria diagnosis and surveillance
Access to timely and accurate diagnostic tests has a significant impact in the management of diseases of global concern such as malaria. While molecular diagnostics satisfy this need effectively in developed c...
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Article
Sub-microliter scale in-gel loop-mediated isothermal amplification (LAMP) for detection of Mycobacterium tuberculosis
Tuberculosis (TB), caused by Mycobacterium tuberculosis, is a common human disease that is prevalent in resource-deprived areas of the world. Current detection techniques for TB require expensive conventional ins...
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Article
Inhibition of on-chip PCR using PDMS–glass hybrid microfluidic chips
In the course of develo** a microfluidic analytical platform incorporating the polymerase chain reaction (PCR) and subsequent capillary electrophoresis (CE) analysis for a variety of bio-assays, we examined ...
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Article
On-chip PCR amplification of genomic and viral templates in unprocessed whole blood
Performing medical diagnosis in microfluidic devices could scale down laboratory functions and reduce the cost for accessible healthcare. The ultimate goal of such devices is to receive a sample of blood, perf...
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Article
On-chip HA/SSCP for the detection of hereditary haemochromatosis
Microfluidics provides a promising tool for meeting the growing demand for high-throughput and low-cost mutation detection technology. With conventional instrumentation, this need is often addressed by the com...
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Article
Open AccessRapid self-assembly of DNA on a microfluidic chip
DNA self-assembly methods have played a major role in enabling methods for acquiring genetic information without having to resort to sequencing, a relatively slow and costly procedure. However, even self-assem...