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Chapter
Dynamics and Structure of the Preprophase Band and the Phragmoplast
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Article
Molecular characterization of Arabidopsis PHO80-like proteins, a novel class of CDKA;1-interacting cyclins
Cyclins are regulatory proteins that interact with cyclin-dependent kinases (CDKs) to control progression through the cell cycle. In Arabidopsis thaliana, 34 cyclin genes have been described, grouped into five di...
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Article
Quantitative cDNA-AFLP analysis for genome-wide expression studies
An improved cDNA-AFLP method for genome-wide expression analysis has been developed. We demonstrate that this method is an efficient tool for quantitative transcript profiling and a valid alternative to microa...
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Article
Novel complexes of cyclin-dependent kinases and a cyclin-like protein from Arabidopsis thaliana with a function unrelated to cell division
Although the majority of cyclin-dependent kinases (CDKs) play a key role in cell cycle progression, recent evidence has shown that CDKs are also implicated in transcription regulation. Here, we describe two Arab...
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Article
Hydrogen peroxide protects tobacco from oxidative stress by inducing a set of antioxidant enzymes
Tolerance against oxidative stress generated by high light intensities or the catalase inhibitor aminotriazole (AT) was induced in intact tobacco plants by spraying them with hydrogen peroxide (H2O2). Stress tol...
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Article
Characterization of microstructure and cell wall components of Arabidopsis thaliana overexpressing cyclin-dependent kinase inhibitor 2
Overexpression of a cyclin-dependent kinase inhibitor (KRP2) caused changes in the general morphology in the leaves of Arabidopsis thaliana. The wild type plant had obovate leaves with entire margins whereas the ...
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Article
Dual action of the active oxygen species during plant stress responses
Adaptation to environmental changes is crucial for plant growth and survival. However, the molecular and biochemical mechanisms of adaptation are still poorly understood and the signaling pathways involved re...
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Article
An Arabidopsis cyclin promoter region is active in transgenic maize plants
We have produced transgenic maize carrying Arabidopsis cyclin promoter and β-glucuronidase chimeric fusion (cycBl;lAt::GUS) by direct DNA uptake into embryogenic cell suspension protoplasts to study the activity ...
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Chapter
Identification of Novel Cell Cycle Genes in Arabidopsis Thaliana
The aim of our research is to understand the molecular basis of cell cycle control in higher plants, in casu in Arabidopsis thaliana, and how intrinsic developmental signals and environmental factors influence ce...
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Article
Factors influencing Agrobacterium tumefaciens-mediated transformation of Artemisia annua L.
Following our previously described Agrobacterium tumefaciens-mediated transformation procedure for Artemisia annua L., we have undertaken several additional experiments to establish the importance of some paramet...
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Article
Cloning and expression analyses of AtMRP4, a novel MRP-like gene from Arabidopsis thaliana
In all organisms glutathione-conjugate transporters (GS-X pumps) mediate the detoxification of a number of xenobiotics by removing them from the cytosol. In addition, GS-X pumps appear to play a role in the p...
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Chapter
Genetic Engineering of Lignin Biosynthesis in Poplar
The removal of lignin in paper manufacture is a toxic and energy-requiring process. For the paper industry and for the environment, it would be very beneficial to process trees with less lignin or with lignin ...
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Chapter
Higher Extractability of Lignin in Poplar (Populus Tremula x P. Alba) by Reducing Cinnamyl Alcohol Dehydrogenase Activity
Cinnamyl alcohol dehydrogenase (CAD) catalyses the last step in the biosynthesis of the lignin precursors, which is the conversion of cinnamaldehydes into cinnamyl alcohols. A poplar (P. trichocarpa x P. deltoide...
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Article
Catalases in plants
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Chapter
Co-suppression of β-1,3-Glucanase Genes in Nicotiana tabacum
Recent studies have demonstrated that introduction of sense gene constructs in plants can result in transgenic plants which express the transgene but also in plants with suppressed levels of transgene expressi...
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Article
Modification of competence for in vitro response to Fusarium oxysporum in tomato cells. II. Effect of the integration of Agrobacterium tumefaciens genes for auxin and cytokinin synthesis
We have studied the effect of a change in the endogenous hormone equilibria on the competence of tomato (Lycopersicon esculentum) cells to defend themselves against the fungal pathogen Fusarium oxysporum f. sp. l...
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Article
Elevated Levels of Superoxide Dismutase Protect Transgenic Plants Against Ozone Damage
To evaluate the feasibility of using engineered antioxidant enzymes as an approach to improve the tolerance of plants to ambient stress, we have constructed transgenlc tobacco plants that overproduce superoxid...
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Article
GTP-binding proteins in plants
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Chapter
Molecular Characterization of Lignin Biosynthesis Genes in Poplar
One of the defense mechanisms of plants to pathogens is the formation of a lignin barrier around the site of infection. The infection is accompanied by an increase in activity of several lignin biosynthetic en...
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Chapter
Phytoalexin Biosynthesis in Chickpea (Cicer Arietinum L.). cDNA Cloning and Regulation of NADPH : Isoflavone Oxidoreductase (IFR)
NADPH: risoflavone oxidoreductase (IFR) is the first soluble enzyme of the pterocarpan specific part of phytoalexin biosynthesis in chickpea. The enzyme was purified to apparent homogeneity. Partial amino acid...
