28 Result(s)
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Corey D. Broeckling
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Open AccessFollicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation
Obesity is a growing concern in human and equine populations, predisposing to metabolic pathologies and reproductive disturbances. Cellular lipid accumulation and mitochondrial dysfunction play an important ro...
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De novo structure generation from tandem mass spectrometry data will advance annotation of unknown metabolomic signals.
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Quantitative analysis of short-chain fatty acids in human plasma and serum by GC–MS
Short-chain fatty acids (SCFAs) are volatile fatty acids produced by gut microbial fermentation of dietary nondigestible carbohydrates. Acetate, propionate, and butyrate SCFA measures are important to clinical...
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Open AccessNon-targeted urine metabolomics and associations with prevalent and incident type 2 diabetes
Better risk prediction and new molecular targets are key priorities in type 2 diabetes (T2D) research. Little is known about the role of the urine metabolome in predicting the risk of T2D. We aimed to use non-...
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Open AccessA novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos
Further refinement of culture media is needed to improve the quality of embryos generated in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the embryo is significantly less t...
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Metabolomics for Rice Grain Quality
The rice grain metabolome encompasses all small molecules present in the grain at a certain time under a specific condition. Grain chemical composition is directly linked to food quality, and a number of analy...
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High-throughput quantitative analysis of phytohormones in sorghum leaf and root tissue by ultra-performance liquid chromatography-mass spectrometry
Plant development, growth, and adaptation to stress are regulated by phytohormones, which can influence physiology even at low concentrations. Phytohormones are chemically grouped according to both structure a...
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Impact of primary carbon sources on microbiome sha** and biotransformation of pharmaceuticals and personal care products
Knowledge of the conditions that promote the growth and activity of pharmaceutical and personal care product (PPCP)-degrading microorganisms within mixed microbial systems are needed to shape microbiomes in bi...
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Open AccessComparison of Machine Learning Algorithms for Predictive Modeling of Beef Attributes Using Rapid Evaporative Ionization Mass Spectrometry (REIMS) Data
Ambient mass spectrometry is an analytical approach that enables ionization of molecules under open-air conditions with no sample preparation and very fast sampling times. Rapid evaporative ionization mass spe...
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Identification of metabolic profiles associated with human exposure to perfluoroalkyl substances
Recent epidemiological studies suggest that human exposure to perfluoroalkyl substances (PFASs) may be associated with type 2 diabetes and other metabolic phenotypes. To gain further insights regarding PFASs e...
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Data Processing for GC-MS- and LC-MS-Based Untargeted Metabolomics
Gas chromatography and liquid chromatography coupled to mass spectrometry are used extensively in untargeted metabolomics, which involves the profiling of small metabolites in biological samples. The complex r...
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Open AccessGlucose challenge metabolomics implicates medium-chain acylcarnitines in insulin resistance
Insulin resistance (IR) predisposes to type 2 diabetes and cardiovascular disease but its causes are incompletely understood. Metabolic challenges like the oral glucose tolerance test (OGTT) can reveal pathoge...
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Ion-neutral Clustering of Bile Acids in Electrospray Ionization Across UPLC Flow Regimes
Bile acid authentic standards were used as model compounds to quantitatively evaluate complex in-source phenomenon on a UPLC-ESI-TOF-MS operated in the negative mode. Three different diameter columns and a cer...
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Open AccessMetabolomics of the tick-Borrelia interaction during the nymphal tick blood meal
The causal agents of Lyme disease in North America, Borrelia burgdorferi and Borrelia mayonii, are transmitted primarily by Ixodes scapularis ticks. Due to their limited metabolic capacity, spirochetes rely on th...
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Open AccessCandida krusei form mycelia along agar surfaces towards each other and other Candida species
Candida krusei has been known to exhibit communal interactions such as pellicle formation and crawling out of nutritional broth. We noticed another possible interaction on agar surface...
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Protocol
Chromatographic Methods to Evaluate Nutritional Quality in Oat
Oats (A. sativa L.) have an important and positive role in human diet and health. The health benefits of oats are attributed to its multifunctional characteristic and nutritional profile, being an important sourc...
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Open AccessProduction of Phloroglucinol, a Platform Chemical, in Arabidopsis using a Bacterial Gene
Phloroglucinol (1,3,5-trihydroxybenzene; PG) and its derivatives are phenolic compounds that are used for various industrial applications. Current methods to synthesize PG are not sustainable due to the requir...
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Non-targeted metabolomics combined with genetic analyses identifies bile acid synthesis and phospholipid metabolism as being associated with incident type 2 diabetes
Identification of novel biomarkers for type 2 diabetes and their genetic determinants could lead to improved understanding of causal pathways and improve risk prediction.
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Large-scale non-targeted metabolomic profiling in three human population-based studies
Non-targeted metabolomic profiling is used to simultaneously assess a large part of the metabolome in a biological sample. Here, we describe both the analytical and computational methods used to analyze a larg...
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Open AccessAnalysis of the metabolome of Anopheles gambiae mosquito after exposure to Mycobacterium ulcerans
Infection with Mycobacterium ulcerans causes Buruli Ulcer, a neglected tropical disease. Mosquito vectors are suspected to participate in the transmission and environmental maintenance of the bacterium. However, ...
