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    Effect of the Freezing Step in the Stability and Bioactivity of Protein-Loaded PLGA Nanoparticles Upon Lyophilization

    The freezing step in lyophilization is the most determinant for the quality of biopharmaceutics. Using insulin as model of therapeutic protein, our aim was to evaluate the freezing effect in the stability and ...

    Pedro Fonte, Fernanda Andrade, Cláudia Azevedo, João Pinto… in Pharmaceutical Research (2016)