27 Result(s)

Article

GDF9^{His209GlnfsTer6/S428T} and GDF9^Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement

Antral follicles consist of an oocyte cumulus complex surrounding by somatic cells, including mural granulosa cells as the inner layer and theca cells as the outsider layer. The communications between oocytes ...

Yuwei Duan, Bing Cai, **g Guo, Chen Wang, Qingyun Mai… in Cell Communication and Signaling (2024)

Article

Clinical application of next generation sequencing-based haplotype linkage analysis in the preimplantation genetic testing for germline mosaicisms

Preimplantation genetic testing (PGT) for monogenic disorders (PGT-M) for germline mosaicism was previously highly dependent on polymerase chain reaction (PCR)-based directed mutation detection combined with l...

Dongjia Chen, Yan Xu, Yu Fu, Yali Wang, Yuliang Liu… in Orphanet Journal of Rare Diseases (2023)

Article

Developmental mRNA m⁵C landscape and regulatory innovations of massive m⁵C modification of maternal mRNAs in animals

m⁵C is one of the longest-known RNA modifications, however, its developmental dynamics, functions, and evolution in mRNAs remain largely unknown. Here, we generate quantitative mRNA m⁵C maps at different stages o...

Jianheng Liu, Tao Huang, Wanying Chen, Chenhui Ding, Tianxuan Zhao… in Nature Communications (2022)

Article

The inconsistency between two major aneuploidy-screening platforms—single-nucleotide polymorphism array and next-generation sequencing—in the detection of embryo mosaicism

In preimplantation genetic testing for aneuploidy (PGT-A), appropriate evaluation of mosaic embryos is important because of the adverse implications of transferring embryos with high-level mosaicism or discard...

Dongjia Chen, Yan Xu, Chenhui Ding, Yali Wang, Yu Fu, Bing Cai, **g Wang… in BMC Genomics (2022)

Article

Multiplex precise base editing in cynomolgus monkeys

Common polygenic diseases result from compounded risk contributed by multiple genetic variants, meaning that simultaneous correction or introduction of single nucleotide variants is required for disease modeli...

Wenhui Zhang, Tomomi Aida, Ricardo C. H. del Rosario… in Nature Communications (2020)

Article

HBB-deficient Macaca fascicularis monkey presents with human β-thalassemia

Yan Huang, Chenhui Ding, Pu** Liang, Duanduan Li, Yu Tang, Wei Meng… in Protein & Cell (2019)

Article

Effect of intrauterine injection of human chorionic gonadotropin before fresh embryo transfer on IVF and ICSI outcomes: a meta-analysis

This analysis was performed to evaluate the effects of intrauterine injection of human chorionic gonadotropin (hCG) before fresh embryo transfer (ET) on the outcomes of in vitro fertilization and intracytoplas...

Wenhui Hou, Gaohui Shi, Bing Cai, Chenhui Ding… in Archives of Gynecology and Obstetrics (2018)

Article

Parental genetic material and oxygen concentration affect hatch dynamics of mouse embryo in vitro

Hatching is crucial for mammalian embryo implantation, since difficulties during this process can lead to implantation failure, ectopic pregnancy and consequent infertility. Despite years of intensive research...

Shaoquan Zhan, Shanbo Cao, Hongzi Du, Yuan Sun… in Reproductive Biology and Endocrinology (2018)

Article

Correction of β-thalassemia mutant by base editor in human embryos

β-Thalassemia is a global health issue, caused by mutations in the HBB gene. Among these mutations, HBB −28 (A>G) mutations is one of the three most common mutations in China and Southeast Asia patients with β-th...

Pu** Liang, Chenhui Ding, Hongwei Sun, **aowei **e, Yanwen Xu… in Protein & Cell (2017)

Article

Glycerol kinase-like proteins cooperate with Pld6 in regulating sperm mitochondrial sheath formation and male fertility

Spermatids undergo the final steps of maturation during spermiogenesis, a process that necessitates extensive rearrangement of organelles such as the mitochondria. Male infertility has been linked to mitochond...

Yuxi Chen, Pu** Liang, Yan Huang, Minyan Li, **ya Zhang, Chenhui Ding… in Cell Discovery (2017)

Article

Effective gene editing by high-fidelity base editor 2 in mouse zygotes

Targeted point mutagenesis through homologous recombination has been widely used in genetic studies and holds considerable promise for repairing disease-causing mutations in patients. However, problems such as...

Pu** Liang, Hongwei Sun, Ying Sun, **ya Zhang, **aowei **e… in Protein & Cell (2017)

Article

The effects of embryo culture media on the birthweight of singletons via fresh or frozen-thawed embryo transfer: a large-scale retrospective study

Embryo culture media used for IVF treatment might affect fetal growth and thus birthweight of the newborns.

Fang Gu, Mingfen Deng, Jun Gao, Zilian Wang, Chenhui Ding… in BMC Pregnancy and Childbirth (2016)

Article

Efficient Production of Gene-Modified Mice using Staphylococcus aureus Cas9

The CRISPR/Cas system is an efficient genome-editing tool to modify genes in mouse zygotes. However, only the Streptococcus pyogenes Cas9 (SpCas9) has been systematically tested for generating gene-modified mice....

**ya Zhang, Pu** Liang, Chenhui Ding, Zhen Zhang, Jianwen Zhou… in Scientific Reports (2016)

Article

Comparison of a xeno-free and serum-free culture system for human embryonic stem cells with conventional culture systems

Elimination of all animal components during derivation and long-term culture of human embryonic stem cells (hESCs) is necessary for future applications of hESCs in clinical cell therapy.

Dan Zhang, Qingyun Mai, Tao Li, Jia Huang, Chenhui Ding… in Stem Cell Research & Therapy (2016)