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Article
Artificial intelligence, drug repurposing and peer review
Can traditional computational analysis and machine learning help compensate for inadequate peer review of drug-repurposing papers in the context of an infodemic?
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Article
Open AccessBlood Biochemistry Analysis to Detect Smoking Status and Quantify Accelerated Aging in Smokers
There is an association between smoking and cancer, cardiovascular disease and all-cause mortality. However, currently, there are no affordable and informative tests for assessing the effects of smoking on the...
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Article
Open AccessIn silico Pathway Activation Network Decomposition Analysis (iPANDA) as a method for biomarker development
Signalling pathway activation analysis is a powerful approach for extracting biologically relevant features from large-scale transcriptomic and proteomic data. However, modern pathway-based methods often fail ...
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Article
Labeling native bacterial RNA in live cells
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Article
Obstructive Sleep Apnea in Patients Undergoing Bariatric Surgery—A Tertiary Center Experience
The patient population that is evaluated for bariatric surgery is characterized by a very high body mass index (BMI). Since obesity is the most important risk factor for obstructive sleep apnea (OSA), sleep di...
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Article
Bacterial charity work leads to population-wide resistance
The emergence of antibiotic-resistant bacterial strains is a growing threat in clinical environments, but the process by which they arise is not well understood. Experiments using a continuous culture of Escheric...
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Article
RNA visualization in live bacterial cells using fluorescent protein complementation
We describe a technique for the detection and localization of RNA transcripts in living cells. The method is based on fluorescent-protein complementation regulated by the interaction of a split RNA-binding pro...
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Article
Plasma placental RNA allelic ratio permits noninvasive prenatal chromosomal aneuploidy detection
Current methods for prenatal diagnosis of chromosomal aneuploidies involve the invasive sampling of fetal materials using procedures such as amniocentesis or chorionic villus sampling and constitute a finite r...
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Article
Comparative genome sequencing of Escherichia coli allows observation of bacterial evolution on a laboratory timescale
We applied whole-genome resequencing of Escherichia coli to monitor the acquisition and fixation of mutations that conveyed a selective growth advantage during adaptation to a glycerol-based growth medium. We ide...
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Article
A bottom-up approach to gene regulation
The ability to construct synthetic gene networks enables experimental investigations of deliberately simplified systems that can be compared to qualitative and quantitative models1,2,3,4,5,6,7,8,9,10,11,12,13,14,
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Article
Haploty** in biomedicine—practical challenges
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Article
Engineered riboregulators enable post-transcriptional control of gene expression
Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both sil...
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Article
Open AccessSimultaneous quantitative and allele-specific expression analysis with real competitive PCR
For a diploid organism such as human, the two alleles of a particular gene can be expressed at different levels due to X chromosome inactivation, gene imprinting, different local promoter activity, or mRNA sta...
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Article
Noise in eukaryotic gene expression
Transcription in eukaryotic cells has been described as quantal1, with pulses of messenger RNA produced in a probabilistic manner2,3. This description reflects the inherently stochastic nature4,5,6,7,8,9 of gene ...
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Protocol
Automated Genoty** Using the DNA MassArray™ Technology
The ongoing progress in establishing a reference sequence as part of the Human Genome Project (1) has revealed a new challenge: the large-scale identification and detection of intraspecies sequence variations, ei...
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Chapter
The Use of MassARRAY Technology for High Throughput Genoty**
This chapter will explore the role of mass spectrometry (MS) as a detection method for genoty** applications and will illustrate how MS evolved from an expert-user-technology to a routine laboratory method i...
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Protocol
Automated Genoty** Using the DNA MassArray™ Technology
The ongoing progress in establishing a reference sequence as part of the Human Genome Project (1) has revealed a new challenge: the large-scale identification and detection of intraspecies sequence variations, ei...
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Article
Construction of a genetic toggle switch in Escherichia coli
It has been proposed1 that gene-regulatory circuits with virtually any desired property can be constructed from networks of simple regulatory elements. These properties, which include multistability and oscillati...
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Chapter
Mass Spectrometry After the Human Genome Project
Almost all of the other speakers in this symposium are using mass spectrometry as a powerful tool to expedite the analysis of problems in biochemistry or cell biology. In contrast, my major focus is genetics. ...
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Article
Symposia lectures
