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  1. No Access

    Article

    Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts

    Application of new genome editing technologies for plant improvement depends on efficient delivery of editing components into plant cells. For editing of canola, which is an important oil crop, CRISPR/Cas9 and Ag...

    Vladimir Sidorov, Dafu Wang, Ervin D. Nagy… in In Vitro Cellular & Developmental Biology … (2022)

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    Article

    “Cell grafting”: a new approach for transferring cytoplasmic or nuclear genome between plants

    A new method based on mixing and wounding of callus tissue was used to transfer plastid or nuclear DNA between cells.

    Vladimir Sidorov, Charles Armstrong, Thomas Ream, Xudong Ye… in Plant Cell Reports (2018)

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    Chapter

    Transient Expression and Stable Transformation of Maize Using Microprojectiles

    High-velocity microprojectiles provide a direct means of introducing DNA into plant cells (Klein et al., 1987, 1988a, 1988b; Wang et al., 1988). In principle, this method could be used to introduce DNA into any a...

    Michael Fromm, Theodore M. Klein, Stephen A. Goff, Brad Roth… in Plant Molecular Biology 2 (1991)