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Article
Practical Characterization Strategies for Comparison, Qualification, and Selection of Cell Viability Detection Methods for Cellular Therapeutic Product Development and Manufacturing
Cellular therapy development and manufacturing has focused on providing novel therapeutic cell-based products for various diseases. The International Organization for Standardization (ISO) has provided guidanc...
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Article
Open AccessDual direction CRISPR transcriptional regulation screening uncovers gene networks driving drug resistance
Pooled CRISPR–Cas9 knock out screens provide a valuable addition to the methods available for novel drug target identification and validation. However, where gene editing is targeted to amplified loci, the res...
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Article
Open AccessCRISPR-Cas9D10A nickase-based genotypic and phenotypic screening to enhance genome editing
The RNA-guided Cas9 nuclease is being widely employed to engineer the genomes of various cells and organisms. Despite the efficient mutagenesis induced by Cas9, off-target effects have raised concerns over the...
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Article
Systematic characterization of deubiquitylating enzymes for roles in maintaining genome integrity
DNA double-strand breaks (DSBs) are perhaps the most toxic of all DNA lesions, with defects in the DNA-damage response to DSBs being associated with various human diseases. Although it is known that DSB repair...
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Article
Open AccessSelective inhibition of microRNA accessibility by RBM38 is required for p53 activity
MicroRNAs (miRNAs) interact with 3′-untranslated regions of messenger RNAs to restrict expression of most protein-coding genes during normal development and cancer. RNA-binding proteins (RBPs) can control the ...
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Article
Open AccessMethylation-mediated silencing and tumour suppressive function of hsa-miR-124 in cervical cancer
A substantial number of microRNAs (miRNAs) is subject to epigenetic silencing in cancer. Although epigenetic silencing of tumour suppressor genes is an important feature of cervical cancer, little is known abo...
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Article
The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis
MicroRNAs (miRNAs) are single-stranded, noncoding RNAs that are important in many biological processes1,2. Although the oncogenic and tumour-suppressive functions of several miRNAs have been characterized, the ro...
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Chapter and Conference Paper
A Genetic Screen Implicates miRNA-372 and miRNA-373 as Oncogenes in Testicular Germ Cell Tumors
Endogenous small RNAs (miRNAs) regulate gene expression by mechanisms conserved across metazoans. While the number of verified human miRNAs is still expanding, only few have been functionally annotated. To per...
