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Article
DNA as the main target in radiotherapy—a historical overview from first isolation to anti-tumour immune response
DNA damage is one of the foremost mechanisms of irradiation at the biological level. After the first isolation of DNA by Friedrich Miescher in the 19th century, the structure of DNA was described by Watson and...
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Article
Open AccessTumor suppressor miR-218 directly targets epidermal growth factor receptor (EGFR) expression in triple-negative breast cancer, sensitizing cells to irradiation
MicroRNA-218 (miR-218) is a key regulator of numerous processes relevant to tumor progression. In the present study, we aimed to characterize the relationship between miR-218 and the Epidermal Growth Factor Re...
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Article
Open AccessThe Musashi RNA-binding proteins in female cancers: insights on molecular mechanisms and therapeutic relevance
RNA-binding proteins have increasingly been identified as important regulators of gene expression given their ability to bind distinct RNA sequences and regulate their fate. Mounting evidence suggests that RNA...
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Protocol
Proteoglycan Expression Studied by MicroRNAs
MicroRNAs are small noncoding RNAs that regulate gene expression at the posttranscriptional level. Proteoglycans are glycoproteins characterized by covalent attachment of a glycosaminoglycan chain, which have ...
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Article
Open AccessCharacterization and dynamics of the soluble immunological microenvironment in melanoma patients undergoing radiotherapy
Malignant melanoma constitutes an aggressive tumor of the skin, the pathogenesis of which is influenced by immunological processes. In this context, the influence of radiotherapy (RT) on inflammatory markers h...
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Article
Open AccessKnockdown of the stem cell marker Musashi-1 inhibits endometrial cancer growth and sensitizes cells to radiation
Endometrial carcinoma is the most common gynecological cancer in Europe. Musashi-1 is known to be a key regulator of endometrial cancer stem cells and a negative prognostic marker. In the present study, we aim...
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Article
Open AccessKnockdown of the prognostic cancer stem cell marker Musashi-1 decreases radio-resistance while enhancing apoptosis in hormone receptor-positive breast cancer cells via p21WAF1/CIP1
While the stem cell marker Musashi-1 (MSI-1) has been identified as a key player in a wide array of malignancies, few findings exist on its prognostic relevance and relevance for cancer cell death and therapy ...
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Article
Open AccessCollagen I triggers directional migration, invasion and matrix remodeling of stroma cells in a 3D spheroid model of endometriosis
Endometriosis is a painful gynecological condition characterized by ectopic growth of endometrial cells. Little is known about its pathogenesis, which is partially due to a lack of suitable experimental models...
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Chapter
Role of Syndecan-1 in Cancer Stem Cells
Syndecan-1 (CD138) is a cell surface heparan sulfate proteoglycan that is frequently misexpressed in cancer. Under physiological and pathological conditions, it is involved in the regulation of diverse process...
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Article
Tumor microenvironmental plasmacytoid dendritic cells contribute to breast cancer lymph node metastasis via CXCR4/SDF-1 axis
Plasmacytoid dendritic cells (PDCs) infiltration into breast cancer tissues is associated with poor prognosis. Also, CXCR4 shows compelling evidences to be exploited by cancer cells to migrate to distant sites...
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Article
MDA-MB-231 breast cancer cell viability, motility and matrix adhesion are regulated by a complex interplay of heparan sulfate, chondroitin−/dermatan sulfate and hyaluronan biosynthesis
Proteoglycans and glycosaminoglycans modulate numerous cellular processes relevant to tumour progression, including cell proliferation, cell-matrix interactions, cell motility and invasive growth. Among the gl...
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Article
Open AccessSyndecan-1 is a novel molecular marker for triple negative inflammatory breast cancer and modulates the cancer stem cell phenotype via the IL-6/STAT3, Notch and EGFR signaling pathways
Inflammatory breast cancer (IBC), a particularly aggressive form of breast cancer, is characterized by cancer stem cell (CSC) phenotype. Due to a lack of targeted therapies, the identification of molecular mar...
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Article
Stereotactic body radiotherapy for liver tumors
This report of the Working Group on Stereotactic Radiotherapy of the German Society of Radiation Oncology (DEGRO) aims to provide a practical guideline for safe and effective stereotactic body radiotherapy (SB...
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Article
Open AccessInfluence of secreted frizzled receptor protein 1 (SFRP1) on neoadjuvant chemotherapy in triple negative breast cancer does not rely on WNT signaling
Triple negative breast cancer (TNBC) is characterized by lack of expression of both estrogen and progesterone receptor as well as lack of overexpression or amplification of HER2. Despite an increased probabili...
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Article
Detektion der Side Population zur Identifizierung von Stammzellen
The quantification of the cellular transport capacity for the DNA staining dye Hoechst 33342 out of the cell is a widely accepted marker for recognition of stem cells. Cells with such an increased level of dye...
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Article
Zellsortierung mit einer geschlossenen, piezobasierten Sortiertechnologie
Das Sortieren von Zellen ist wichtig für die Klonierung von Zellen oder zur Anreicherung von seltenen Zellpopulationen. Ein neuer Zellsorter bietet eine hohe Leistung und Nutzerfreundlichkeit.
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Article
Open AccessAssociation of telomerase activity with radio- and chemosensitivity of neuroblastomas
Telomerase activity compensates shortening of telomeres during cell division and enables cancer cells to escape senescent processes. It is also supposed, that telomerase is associated with radio- and chemoresi...
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Article
Open AccessSelective regain of egfr gene copies in CD44+/CD24-/lowbreast cancer cellular model MDA-MB-468
Increased transcription of oncogenes like the epidermal growth factor receptor (EGFR) is frequently caused by amplification of the whole gene or at least of regulatory sequences. Aim of this study was to pinpo...
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Article
Multicentric investigation of ionising radiation-induced cell death as a predictive parameter of individual radiosensitivity
In the present study, the predictive value of ionising radiation (IR)-induced cell death was tested in peripheral blood lymphocytes (PBLs) and their corresponding Epstein-Barr virus-transformed lymphoblastoid ...
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Article
Plastidic (Pho1-type) phosphorylase isoforms in potato (Solanum tuberosum L.) plants: expression analysis and immunochemical characterization
Higher plants contain two types of phosphorylase (EC 2.4.1.1). One type is plastidic (Pho1) and the other resides in the cytosol (Pho2). For Solanum tuberosum L., two highly homologous Pho1-type sequences (desig...
