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  1. Article

    Open Access

    Combinatorial approach for improved cyanidin 3-O-glucoside production in Escherichia coli

    Multi-monocistronic and multi-variate vectors were designed, built, and tested for the improved production of cyanidin 3-O-glucoside (C3G) in Escherichia coli BL21 (DE3). The synthetic bio-parts were designed in ...

    Biplav Shrestha, Ramesh Prasad Pandey, Sumangala Darsandhari… in Microbial Cell Factories (2019)

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  2. No Access

    Chapter

    Microbial Modifications of Flavonols

    Development of microbial cell factories via application of synthetic biology, protein engineering for metabolic engineering has revolutionized the maximum use of microbial consortium for biosynthesis and struc...

    Prakash Parajuli, Biplav Shrestha, Jae Kyung Sohng… in Pharmaceuticals from Microbes (2019)

  3. No Access

    Chapter

    Engineering Streptomyces peucetius for Doxorubicin and Daunorubicin Biosynthesis

    Doxorubicin and daunorubicin are notable members of the type II polyketide synthase family and clinically important cancer chemotherapeutic agents and are produced by a mutant strain S. peucetius ATCC 27952. They...

    Biplav Shrestha, Anaya Raj Pokhrel, Sumangala Darsandhari… in Pharmaceuticals from Microbes (2019)

  4. No Access

    Article

    Heterologous production of clavulanic acid intermediates in Streptomyces venezuelae

    Heterologous expression can enhance production of diverse secondary metabolites by redirecting precursor pools towards compound of interest. In this study, Streptomyces venezuelae YJ028 was utilized as the het...

    Biplav Shrestha, Dipesh Dhakal… in Biotechnology and Bioprocess Engineering (2017)

  5. No Access

    Article

    Enhanced production of nargenicin A1 and creation of a novel derivative using a synthetic biology platform

    Nargenicin A1, an antibacterial produced by Nocardia sp. CS682 (KCTC 11297BP), demonstrates effective activity against various Gram-positive bacteria. Hence, we attempted to enhance nargenicin A1 production by ut...

    Dipesh Dhakal, Amit Kumar Chaudhary… in Applied Microbiology and Biotechnology (2016)