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Article
Low phytate soybean: next generation metabolic engineering using CRISPR-Cas 9 genome editing technology
Next-generation metabolic engineering widens the possibility of using plants as biofactories for the mass production of metabolites. CRISPR/Cas9 system is the newest and the most widely adapted genome editing ...
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Article
Nutritional supremacy of pearl- and foxtail millets: assessing the nutrient density, protein stability and shelf-life of flours in millets and cereals for develo** nutri-stable foods
Flour of millets is nutritionally better, as compared to cereals, but has problem of low shelf-life. Here, we analyzed the nutritional density, and shelf-life of flours of millets (pearl millet, foxtail millet...
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Article
A Novel Continuous Enzyme Coupled Colorimetric Assay for Phospholipase A2 and its Application in the Determination of Catalytic Activity of Oil-Body–Associated Oleosin Protein
The present work reports a novel, continuous, specific, and colorimetric coupled method for the assay of phospholipase A2 (PLA2), which involves use of acyl-CoA synthetase (ACS) as coupling enzyme and Dilinoleoyl...
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Article
Effects of gamma irradiation on soybean oil stability by enhancing tocopherol content in soybean
Soybean oil has high contents of polyunsaturated fatty acids and hence susceptible to deterioration due to the presence of lipase and lipoxygenase (LOX) enzymes. These enzymes cause the severe impact in the re...
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Article
Rancidity Matrix: Development of Biochemical Indicators for Analysing the Kee** Quality of Pearl Millet Flour
Millets are known for its nutritional excellence because of its high nutrient density. Among all the millets, pearl millet is considered the most important nutricereals, having 3–8% lipids. Rancidity or off-od...
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Article
Anthocyanin fingerprinting and dynamics in differentially pigmented exotic soybean genotypes using modified HPLC–DAD method
More than as a phenotypic marker for breeding, seed coat colour of soybean is gaining momentum as a nutraceutical marker owing to the multitude of medicinal effects provided by anthocyanins. The acute obstacle...
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Article
GFP tagging based method to analyze the genome editing efficiency of CRISPR/Cas9-gRNAs through transient expression in N. benthamiana
The CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR—associated proteins 9) is simple and highly efficient technology applied to functional studies of genes and genetic crop improv...
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Article
‘AGRODATE’: a rapid Agrobacterium-mediated transient expression tool for gene function analysis in leaf discs
Transient gene expression utilizing syringe mediated agro infiltration offers a simple yet efficient technique for various transgenic applications. Although soybean is commonly used as a model plant for functi...
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Article
Expression profiling and in silico homology modeling of Inositol pentakisphosphate 2-kinase, a potential candidate gene for low phytate trait in soybean
Low phytate soybeans are desirable both from a nutritional and economic standpoint. Inositol 1, 3, 4, 5, 6-pentakisphosphate 2-kinase (IPK1), optimizes the metabolic flux of phytate generation in soybean and thus...
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Article
Study of subcellular localization of Glycine max γ-tocopherol methyl transferase isoforms in N. benthamiana
Gamma-tocopherol methyltransferase (γ-TMT) converts γ-toc to α-toc—the rate limiting step in toc biosynthesis. Sequencing results revealed that the coding regions of γ-TMT1 and γ-TMT3 were strongly similar to eac...
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Article
Black Carrot (Daucus carota ssp.) and Black Soybean (Glycine max (L.) Merr.) Anthocyanin Extract: A Remedy to Enhance Stability and Functionality of Fruit Juices by Copigmentation
Application of extracted black carrot anthocyanins (BCA) and black soybean anthocyanins (BSA) as colour enhancers was demonstrated by copigmenting with heat sensitive anthocyanins from plum, pomegranate, straw...
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Article
Functional characterization of GmITPK (myo-inositol: 1, 3, 4 tris phosphate 5/6 kinase) isoforms—‘so different yet so similar’
The biochemical basis behind the preferential presence and action of GmITPKs (myo-inositol—1, 3, 4 tris phosphate 5/6 kinases) in regulating the phosphorous flux towards phytate synthesis in soybean is an importa...
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Article
Conserved miRNAs modulate the expression of potential transcription factors of isoflavonoid biosynthetic pathway in soybean seeds
Despite the significant importance of soybean isoflavone, the regulatory mechanism of miRNAs during its biosynthesis is highly unexplored. In the present work, nine existing miRNAs along with their ten corresp...
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Article
Open AccessSeed targeted RNAi-mediated silencing of GmMIPS1 limits phytate accumulation and improves mineral bioavailability in soybean
Phytic acid (PA), the major phosphorus reserve in soybean seeds (60–80%), is a potent ion chelator, causing deficiencies that leads to malnutrition. Several forward and reverse genetics approaches have ever si...
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Article
Valorisation of black carrot pomace: microwave assisted extraction of bioactive phytoceuticals and antioxidant activity using Box–Behnken design
The present study compares three methods viz. microwave assisted extraction (MAE), ultrasonic-assisted extraction (UAE) and conventional solvent extraction (CSE) for extraction of phenolic compounds from black...
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Article
Improved Agrobacterium tumefaciens-mediated transformation of soybean [Glycine max (L.) Merr.] following optimization of culture conditions and mechanical techniques
In the present study, Agrobacterium tumefaciens-mediated transformation of Glycine max (L.) Merr. (soybean) cv. DS-9712 using half-seed explants was optimized for eight different parameters, including seed imbibi...
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Article
Molecular characterization, modeling, and docking analysis of late phytic acid biosynthesis pathway gene, inositol polyphosphate 6-/3-/5-kinase, a potential candidate for develo** low phytate crops
The coding sequence of inositol polyphosphate 6-/3-/5-kinase (GmIPK2) gene was identified and cloned from popular Indian soybean cultivar Pusa-16. The clone was predicted to encode 279 amino acids long, 30.97 kDa...
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Article
Molecular cloning and functional analysis of the promoter of γ-Tocopherol Methyl Transferase (γ-TMT) gene of soybean (Glycine max)
γ-Tocopherol methyl transferase (γ-TMT) (EC 2.1.1.95) is the key enzyme of the tocopherol biosynthetic pathway that determines the α-tocopherol concentration in plants. The overexpression of γ-TMT has been a succ...
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Article
Molecular modeling and in silico characterization of GmABCC5: a phytate transporter and potential target for low-phytate crops
Designing low-phytate crops without affecting the developmental process in plants had led to the identification of ABCC5 gene in soybean. The GmABCC5 gene was identified and a partial gene sequence was cloned fro...
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Article
Characterization and molecular modeling of Inositol 1,3,4 tris phosphate 5/6 kinase-2 from Glycine max (L) Merr.: comprehending its evolutionary conservancy at functional level
Soybean genome encodes a family of four inositol 1,3,4 trisphosphate 5/6 kinases which belong to the ATP-GRASP group of proteins. Inositol 1,3,4 trisphosphate kinase-2 (GmItpk2), catalyzing the ATP-dependent phos...
