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Article
Open AccessPolysaccharide composition during cotton seed fibre development: temporal differences between species and in different seasons
Cotton seed fibres are long single-celled epidermal trichomes that first appear on the surface of the ovule at anthesis and then elongate rapidly over a period of 15–25 days until a secondary cell wall (SCW) b...
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Article
Open AccessArabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments
Seagrasses evolved from monocotyledonous land plants that returned to the marine habitat. This transition was accomplished by substantial changes in cell wall composition, revealing habitat-driven adaption to ...
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Article
A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids
Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational protein modifications and glycolipid and polysaccharide biosynthesis. Most of the...
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Article
Open AccessTranscriptome analysis provides insights into xylogenesis formation in Moso bamboo (Phyllostachys edulis) shoot
Maturation-related changes in cell wall composition and the molecular mechanisms underlying cell wall changes were investigated from the apical, middle and basal segments in moso bamboo shoot (MBS). With matur...
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Article
Open AccessProteomics informed by transcriptomics for characterising differential cellular susceptibility to Nelson Bay orthoreovirus infection
Nelson Bay orthoreovirus (NBV) is a fusogenic bat borne virus with an unknown zoonotic potential. Previous studies have shown that NBV can infect and replicate in a wide variety of cell types derived from thei...
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Protocol
Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings
The Golgi apparatus is an essential component in the plant secretory pathway. The enrichment of Golgi membranes from plant tissue is fundamental to the study of this structurally complex organelle. The utiliza...
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Article
The plant secretory pathway seen through the lens of the cell wall
Secretion in plant cells is often studied by looking at well-characterised, evolutionarily conserved membrane proteins associated with particular endomembrane compartments. Studies using live cell microscopy a...
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Article
Open AccessMASTR-MS: a web-based collaborative laboratory information management system (LIMS) for metabolomics
An increasing number of research laboratories and core analytical facilities around the world are develo** high throughput metabolomic analytical and data processing pipelines that are capable of handling hu...
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Article
Nitrogen assimilation system in maize is regulated by developmental and tissue-specific mechanisms
We found metabolites, enzyme activities and enzyme transcript abundances vary significantly across the maize lifecycle, but weak correlation exists between the three groups. We identified putative genes regula...
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Article
Open AccessThe cnidarian Hydractinia echinata employs canonical and highly adapted histones to pack its DNA
Cnidarians are a group of early branching animals including corals, jellyfish and hydroids that are renowned for their high regenerative ability, growth plasticity and longevity. Because cnidarian genomes are ...
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Article
Open AccessDe novo transcriptome assembly and analysis of differentially expressed genes of two barley genotypes reveal root-zone-specific responses to salt exposure
Plant roots are the first organs sensing and responding to salinity stress, manifested differentially between different root types, and also at the individual tissue and cellular level. High genetic diversity ...
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Article
Open AccessMass spectrometry imaging for plant biology: a review
Mass spectrometry imaging (MSI) is a develo** technique to measure the spatio-temporal distribution of many biomolecules in tissues. Over the preceding decade, MSI has been adopted by plant biologists and ap...
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Article
A tandem liquid chromatography–mass spectrometry (LC–MS) method for profiling small molecules in complex samples
Liquid chromatography–mass spectrometry (LC–MS) methods using either aqueous normal phase (ANP) or reversed phase (RP) columns are routinely used in small molecule or metabolomic analyses. These stationary pha...
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Article
Gaining insight into cell wall cellulose macrofibril organisation by simulating microfibril adsorption
One of the most important interactions within the paracrystalline matrix of the plant cell wall occurs between cellulose microfibrils to allow for the formation of larger diameter macrofibrils. Here, we have u...
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Article
Open AccessProteomic analysis of Pteropus alecto kidney cells in response to the viral mimic, Poly I:C
Bats are recognised as an important reservoir for a number of highly pathogenic zoonotic viruses. While many of these viruses cause severe and often fatal disease in humans, bats are able to coexist with these...
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Article
Open AccessArabidopsis leucine-rich repeat extensin (LRX) proteins modify cell wall composition and influence plant growth
Leucine-rich repeat extensins (LRXs) are extracellular proteins consisting of an N-terminal leucine-rich repeat (LRR) domain and a C-terminal extensin domain containing the typical features of this class of st...
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Article
Open AccessSpatial gradients in cell wall composition and transcriptional profiles along elongating maize internodes
The elongating maize internode represents a useful system for following development of cell walls in vegetative cells in the Poaceae family. Elongating internodes can be divided into four developmental zones, ...
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Protocol
LC-MS Profiling to Link Metabolic and Phenotypic Diversity in Plant Map** Populations
Numerous studies have revealed the extent of genetic, phenotypic, and metabolic variation between different plant cultivars/varieties. We present a specialized protocol for large-scale targeted and untargeted ...
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Article
Exploratory analysis of high-throughput metabolomic data
In order to make sense of the sheer volume of metabolomic data that can be generated using current technology, robust data analysis tools are essential. We propose the use of the growing self-organizing map (G...
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Article
An enzyme activity capable of endotransglycosylation of heteroxylan polysaccharides is present in plant primary cell walls
Heteroxylans in the plant cell wall have been proposed to have a role analogous to that of xyloglucans or heteromannans, forming growth-restraining networks by interlocking cellulose microfibrils. A xylan endo...
