Polyamines
Methods and Protocols
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21 Result(s)
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Antonio F. Tiburcio
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Book
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Protocol
Analysis of Polyamines Conjugated with Hydroxycinnamoyl Acids by High-Performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry
Polyamines conjugated with hydroxycinnamic acids are phenolic compounds, which are widespread in the plant kingdom playing important roles in development and defence responses. This chapter describes the metho...
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Protocol
Generation of EMS-Mutagenized Populations of Arabidopsis thaliana for Polyamine Genetics
In the recent years, genetic engineering of polyamine biosynthetic genes has provided evidence for their involvement in plant stress responses and different aspects of plant development. Such approaches are be...
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Protocol
Determination of S-Adenosylmethionine Decarboxylase Activity in Plants
The synthesis of spermidine, spermine and thermospermine requires the addition of aminopropyl groups from decarboxylated S-adenosyl-methionine (dSAM). The synthesis of dSAM is catalyzed by S-adenosylmethionine de...
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Determination of Posttranslational Modifications by 2D PAGE: Applications to Polyamines
Polyamines not only affect transcription and translation but also may induce a number of posttranslational modifications. The identification of polyamine-induced posttranslational modifications can be performe...
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Determination of Arginine and Ornithine Decarboxylase Activities in Plants
In plants, putrescine is synthesized directly from the decarboxylation of ornithine and/or by the alternative arginine decarboxylase pathway. The prevalence of one or the other depends on the tissue and stress...
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Protocol
Potential Applications of Polyamines in Agriculture and Plant Biotechnology
The polyamines putrescine, spermidine and spermine have been implicated in a myriad of biological functions in many organisms. Research done during the last decades has accumulated a large body of evidence dem...
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Article
Polyamines: Emerging Hubs Promoting Drought and Salt Stress Tolerance in Plants
Environmental stress is increasingly wearing down crop productivity. As a consequence, a major priority of plant research is to get deeper insights on tolerance mechanisms, providing solutions with the generat...
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Chapter
Drought Stress Tolerance in Relation to Polyamine Metabolism in Plants
Polyamines (PAs) are small protonated molecules with amine groups that are found in most living organisms. In plants, most abundant polyamines are putrescine, spermidine, spermine, and its isomer thermospermin...
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Polyamine Biosynthesis Engineering as a Tool to Improve Plant Resistance to Abiotic Stress
Polyamines (PAs) are small polycationic molecules which are present in all living organisms. PAs have been involved in a wide array of metabolic plant processes, extending from development to stress protection...
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Genetic Engineering Strategies for Abiotic Stress Tolerance in Plants
Crop plants are affected by a variety of abiotic stresses such as salinity, drought, extreme temperatures, and oxidative stress and cause a significant yield loss (more than 50 %). In the near future, these ab...
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Article
The roles of polyamines during the lifespan of plants: from development to stress
Compelling evidence indicates that free polyamines (PAs) (mainly putrescine, spermidine, spermine, and its isomer thermospermine), some PA conjugates to hydroxycinnamic acids, and the products of PA oxidation ...
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Article
Open AccessCopper-containing amine oxidases contribute to terminal polyamine oxidation in peroxisomes and apoplast of Arabidopsis thaliana
Polyamines (PAs) are oxidatively deaminated at their primary or secondary amino-groups by copper-containing amine oxidases (CuAOs) or FAD-dependent amine oxidases (PAOs), respectively. Both enzymes have long b...
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Article
Polyamines: molecules with regulatory functions in plant abiotic stress tolerance
Early studies on plant polyamine research pointed to their involvement in responses to different environmental stresses. During the last few years, genetic, transcriptomic and metabolomic approaches have unrav...
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Involvement of polyamines in plant response to abiotic stress
Environmental stresses are the major cause of crop loss worldwide. Polyamines are involved in plant stress responses. However, the precise role(s) of polyamine metabolism in these processes remain ill-defined....
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Integrated Molecular Analysis of the Polyamine Metabolic Pathway in Abiotic Stress Signalling
Land plants experience constant fluctuations in the availability of water, thus they have evolved adaptive features to mine and absorb water through the root system, to prevent excessive transpiration water lo...
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Chapter
Polyamines Determination by TLC and HPLC
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Article
Improved plant regeneration in maize callus cultures after pretreatment with dl-alpha-difluoromethylarginine
The influence of a three-month-long pretreatment with dl-alpha-difluoromethylarginine (DFMA), an irreversible suicide inhibitor of arginine decarboxylase activity (ADC; EC 4.1.1.19), on plant regeneration, protei...
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Changes in Polyamines Related with Pruning as a Method for Rejuvenation in Filbert
Polyamine titer in leaves taken from mature and severely pruned trees (two successive treatments) of filbert (Corylus avellana L. cvs Gironella and Negreta) was investigated in order to determine the possible rel...
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Article
Morphogenesis in tobacco subepidermal cells: Putrescine as marker of root differentiation
We have used the tobacco thin cell layer ‘in vitro’ system to evaluate changes in polyamine titers as correlated with root differentiation and with variations in external pH during culture. We show that root d...
