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Analysis of Polyamines Conjugated with Hydroxycinnamoyl Acids by High-Performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry

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Polyamines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1694))

Abstract

Polyamines conjugated with hydroxycinnamic acids are phenolic compounds, which are widespread in the plant kingdom playing important roles in development and defence responses. This chapter describes the methodology employed to analyze these phenolamides in plant material by liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-MS-MS). These compounds are not always in sufficient concentration in plant tissues for analysis by more conventional methods such as UV detection of HPLC. Owing to their particular molecular structure, they cannot be analyzed as free polyamines. Thus, described herein is an extraction method for hydroxycinnamic acid amides in plant tissues such as leaves, and their analysis by LC-MS-MS, including identification and quantification protocols.

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Acknowledgment

The authors are thankful to the Scientific Technical Services from University of Barcelona. The research team belongs to the Natural Products Group (2016-SGR-920 Generalitat de Catalunya).

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Authors and Affiliations

  1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII 27-31, 08028, Barcelona, Spain

    Laura Torras-Claveria

  2. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Avda. Joan XXIII 27-31, 08028, Barcelona, Spain

    Jaume Bastida, Francesc Viladomat & Antonio F. Tiburcio

Authors
  1. Laura Torras-Claveria
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  2. Jaume Bastida
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  3. Francesc Viladomat
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  4. Antonio F. Tiburcio
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Corresponding author

Correspondence to Antonio F. Tiburcio .

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Editors and Affiliations

  1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Rubén Alcázar

  2. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Antonio F. Tiburcio

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Torras-Claveria, L., Bastida, J., Viladomat, F., Tiburcio, A.F. (2018). Analysis of Polyamines Conjugated with Hydroxycinnamoyl Acids by High-Performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry. In: Alcázar, R., Tiburcio, A. (eds) Polyamines. Methods in Molecular Biology, vol 1694. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7398-9_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7398-9_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7397-2

  • Online ISBN: 978-1-4939-7398-9

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