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Article
Open AccessMycobacterium tuberculosis β-lactamase variant reduces sensitivity to ampicillin/avibactam in a zebrafish-Mycobacterium marinum model of tuberculosis
The β-lactamase of Mycobacterium tuberculosis, BlaC, hydrolyzes β-lactam antibiotics, hindering the use of these antibiotics for the treatment of tuberculosis. Inhibitors, such as avibactam, can reversibly inhibi...
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Article
Open AccessThe adapter protein Myd88 plays an important role in limiting mycobacterial growth in a zebrafish model for tuberculosis
Tuberculosis (TB) is the most prevalent bacterial infectious disease in the world, caused by the pathogen Mycobacterium tuberculosis (Mtb). In this study, we have used Mycobacterium marinum (Mm) infection in zebr...
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Article
Open AccessDeficiency in the autophagy modulator Dram1 exacerbates pyroptotic cell death of Mycobacteria-infected macrophages
DNA damage regulated autophagy modulator 1 (DRAM1) is a stress-inducible regulator of autophagy and cell death. DRAM1 has been implicated in cancer, myocardial infarction, and infectious diseases, but the mole...
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Article
Open AccessInfection and RNA-seq analysis of a zebrafish tlr2 mutant shows a broad function of this toll-like receptor in transcriptional and metabolic control and defense to Mycobacterium marinum infection
The function of Toll-like receptor 2 (TLR2) in host defense against pathogens, especially Mycobacterium tuberculosis (Mtb) is poorly understood. To investigate the role of TLR2 during mycobacterial infection, we ...
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Article
Open AccessAspergillus fumigatus establishes infection in zebrafish by germination of phagocytized conidia, while Aspergillus niger relies on extracellular germination
Among opportunistically pathogenic filamentous fungi of the Aspergillus genus, Aspergillus fumigatus stands out as a drastically more prevalent cause of infection than others. Utilizing the zebrafish embryo model...
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Article
Adverse outcome pathways: opportunities, limitations and open questions
Adverse outcome pathways (AOPs) are a recent toxicological construct that connects, in a formalized, transparent and quality-controlled way, mechanistic information to apical endpoints for regulatory purposes....
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Article
Open AccessExpression and regulation of drug transporters in vertebrate neutrophils
There remains a need to identify novel pro-resolution drugs for treatment of inflammatory disease. To date, there are no neutrophil-specific anti-inflammatory treatments in clinical use, perhaps due to our lac...
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Article
Open AccessThe chemokine receptor CXCR4 promotes granuloma formation by sustaining a mycobacteria-induced angiogenesis programme
CXC chemokine receptor 4 plays a critical role in chemotaxis and leukocyte differentiation. Furthermore, there is increasing evidence that links this receptor to angiogenesis. Using the well-established zebraf...
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Article
Open AccessProtection and pathology in TB: learning from the zebrafish model
Zebrafish has earned its place among animal models of tuberculosis. Its natural pathogen, Mycobacterium marinum, shares major virulence factors with the human pathogen Mycobacterium tuberculosis. In adult zebrafi...
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Protocol
Imaging of Human Cancer Cell Proliferation, Invasion, and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model
The xenograft model, using the early life stages of the zebrafish, allows imaging of tumor cell behavior both on a single cell and whole organism level, over time, within a week. This robust and reproducible a...
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Article
Open AccessCommon and specific downstream signaling targets controlled by Tlr2 and Tlr5 innate immune signaling in zebrafish
Although the responses to many pathogen associated molecular patterns (PAMPs) in cell cultures and extracted organs are well characterized, there is little known of transcriptome responses to PAMPs in whole or...
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Article
Open AccessAnalysis of RNAseq datasets from a comparative infectious disease zebrafish model using GeneTiles bioinformatics
We present a RNA deep sequencing (RNAseq) analysis of a comparison of the transcriptome responses to infection of zebrafish larvae with Staphylococcus epidermidis and Mycobacterium marinum bacteria. We show how o...
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Protocol
RNA Sequencing of FACS-Sorted Immune Cell Populations from Zebrafish Infection Models to Identify Cell Specific Responses to Intracellular Pathogens
The zebrafish (Danio rerio) is increasingly used as a model for studying infectious diseases. This nonmammalian vertebrate host, which is transparent at the early life stages, is especially attractive for live im...
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Article
Open AccessParallel deep transcriptome and proteome analysis of zebrafish larvae
Sensitivity and throughput of transcriptomic and proteomic technologies have advanced tremendously in recent years. With the use of deep sequencing of RNA samples (RNA-seq) and mass spectrometry technology for...
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Article
Open AccessMicroRNA-146 function in the innate immune transcriptome response of zebrafish embryos to Salmonella typhimurium infection
MicroRNAs (miRNAs) have recently been shown to play important roles in development of the immune system and in fine-tuning of immune responses. Human miR-146 family members are known as inflammation-inducible ...
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Article
Open AccessA zebrafish high throughput screening system used for Staphylococcus epidermidisinfection marker discovery
Staphylococcus epidermidis bacteria are a major cause of biomaterial-associated infections in modern medicine. Yet there is little known about the host responses against this normally innocent bacterium in the co...
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Article
Function of the HD-Zip I gene Oshox22 in ABA-mediated drought and salt tolerances in rice
Oshox22 belongs to the homeodomain-leucine zipper (HD-Zip) family I of transcription factors, most of which have unknown functions. Here we show that the expression of Oshox22 is strongly induced by salt stress, ...
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Chapter and Conference Paper
Spatio-temporal Modeling and Simulation of Mycobacterium Pathogenesis Using Petri Nets
Computational modeling of biological systems is becoming increasingly important in the endeavors to better understand complex biological behavior. It enables researchers to perform computerized simulations usi...
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Article
Open AccessComparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tarda
The zebrafish embryo is an important in vivo model to study the host innate immune response towards microbial infection. In most zebrafish infectious disease models, infection is achieved by micro-injection of ba...
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Article
Open AccessRapid screening of innate immune gene expression in zebrafish using reverse transcription - multiplex ligation-dependent probe amplification
With the zebrafish increasingly being used in immunology and infectious disease research, there is a need for efficient molecular tools to evaluate immune gene expression in this model species. RT-MLPA (revers...