8 Result(s)
within
Andrew P Hutchins
Science, Humanities and Social Sciences, multidisciplinary
-
Article
Open AccessVGLL1 cooperates with TEAD4 to control human trophectoderm lineage specification
In contrast to rodents, the mechanisms underlying human trophectoderm and early placenta specification are understudied due to ethical barriers and the scarcity of embryos. Recent reports have shown that human...
-
Article
Open AccessIdentifying transposable element expression dynamics and heterogeneity during development at the single-cell level with a processing pipeline scTE
Transposable elements (TEs) make up a majority of a typical eukaryote’s genome, and contribute to cell heterogeneity in unclear ways. Single-cell sequencing technologies are powerful tools to explore cells, ho...
-
Article
Open AccessJMJD3 acts in tandem with KLF4 to facilitate reprogramming to pluripotency
The interplay between the Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) and transcriptional/epigenetic co-regulators in somatic cell reprogramming is incompletely understood. Here, we demonstrate that the hist...
-
Article
Open AccessPluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2
Oct4, along with Sox2 and Klf4 (SK), can induce pluripotency but structurally similar factors like Oct6 cannot. To decode why Oct4 has this unique ability, we compare Oct4-binding, accessibility patterns and t...
-
Article
Open AccessAn alternative CTCF isoform antagonizes canonical CTCF occupancy and changes chromatin architecture to promote apoptosis
CTCF plays key roles in gene regulation, chromatin insulation, imprinting, X chromosome inactivation and organizing the higher-order chromatin architecture of mammalian genomes. Previous studies have mainly fo...
-
Article
Open AccessTransposable elements are regulated by context-specific patterns of chromatin marks in mouse embryonic stem cells
The majority of mammalian genomes are devoted to transposable elements (TEs). Whilst TEs are increasingly recognized for their important biological functions, they are a potential danger to genomic stability a...
-
Article
Open AccessPCGF5 is required for neural differentiation of embryonic stem cells
Polycomb repressive complex 1 (PRC1) is an important regulator of gene expression and development. PRC1 contains the E3 ligases RING1A/B, which monoubiquitinate lysine 119 at histone H2A (H2AK119ub1), and has ...
-
Article
Open AccessA sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes
Reprogramming has been shown to involve EMT–MET; however, its role in cell differentiation is unclear. We report here that in vitro differentiation of hESCs to hepatic lineage undergoes a sequential EMT–MET with ...
