10 Result(s)
within
Andrea Barbero
English
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Reference Work Entry In depth
Cells for Cartilage Regeneration
Cartilage, at all sites of our body, has limited intrinsic capacity to repair. There are numerous methods to use when repairing cartilage, and the preferred method depends on the location of the cartilage defe...
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Article
Hyperphysiological compression of articular cartilage induces an osteoarthritic phenotype in a cartilage-on-a-chip model
Owing to population aging, the social impact of osteoarthritis (OA)—the most common musculoskeletal disease—is expected to increase dramatically. Yet, therapy is still limited to palliative treatments or surgi...
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Living Reference Work Entry In depth
Cells for Cartilage Regeneration
Cartilage, at all sites of our body, has limited intrinsic capacity to repair. There are numerous methods to use when repairing cartilage, and the preferred method depends on the location of the cartilage defe...
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Article
Open AccessNotochordal cell conditioned medium (NCCM) regenerates end-stage human osteoarthritic articular chondrocytes and promotes a healthy phenotype
Notochordal cell conditioned medium (NCCM) derived from non-chondrodystrophic dogs has pro-anabolic and anti-catabolic effects upon nucleus pulposus (NP) cells. Here, for the first time, we assessed the abilit...
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Protocol
Engineering Small-Scale and Scaffold-Based Bone Organs via Endochondral Ossification Using Adult Progenitor Cells
Bone development, growth, and repair predominantly occur through the process of endochondral ossification, characterized by remodelling of cartilaginous templates. The same route efficiently supports engineeri...
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Article
Substrate elasticity modulates TGF beta stimulated re-differentiation of expanded human articular chondrocytes
Substrate elasticity has emerged as important biomaterial design parameter. In particular, it has been reported that on soft substrates (~4 kPa) freshly isolated porcine chondrocytes better maintain their phen...
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Article
Dynamic formation of oriented patches in chondrocyte cell cultures
Growth factors have a significant impact not only on the growth dynamics but also on the phenotype of chondrocytes (Barbero et al. in J. Cell. Phys. 204:830–838, 2005). In particular, as chondrocytes approach con...
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Article
Open AccessAnabolic and catabolic responses of human articular chondrocytes to varying oxygen percentages
Oxygen is a critical parameter proposed to modulate the functions of chondrocytes ex-vivo as well as in damaged joints. This article investigates the effect of low (more physiological) oxygen percentage on the bi...
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Article
A novel implantation technique for engineered osteo-chondral grafts
We present a novel method to support precise insertion of engineered osteochondral grafts by pulling from the bone layer, thereby minimizing iatrogenic damage associated with direct manipulation of the cartila...
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Protocol
Human Articular Chondrocytes Culture
In this chapter, we describe a strategy to expand adult human articular chondrocytes in monolayer while maintaining high post-expansion chondrogenic capacity. This method relies on the use of a specific growth...
