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Article
Open AccessGene repositioning within the cell nucleus is not random and is determined by its genomic neighborhood
Heterochromatin has been reported to be a major silencing compartment during development and differentiation. Prominent heterochromatin compartments are located at the nuclear periphery and inside the nucleus ...
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Article
Open AccessHistone acetylation controls the inactive X chromosome replication dynamics
In mammals, dosage compensation between male and female cells is achieved by inactivating one female X chromosome (**). Late replication of ** was proposed to be involved in the maintenance of its silenced sta...
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Protocol
FISH on 3D Preserved Bovine and Murine Preimplantation Embryos
Fluorescence in situ hybridization (FISH) is a commonly used technique for the visualization of whole chromosomes or subchromosomal regions, such as chromosome arms, bands, centromeres, or single gene loci. FI...
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Article
Open AccessCommon themes and cell type specific variations of higher order chromatin arrangements in the mouse
Similarities as well as differences in higher order chromatin arrangements of human cell types were previously reported. For an evolutionary comparison, we now studied the arrangements of chromosome territorie...
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Article
Three-dimensional arrangements of centromeres and telomeres in nuclei of human and murine lymphocytes
The location of centromeres and telomeres was studied in human and mouse lymphocyte nuclei (G0) employing 3D-FISH, confocal microscopy, and quantitative image analysis. In both human and murine lymphocytes, mo...
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Article
Non-random radial higher-order chromatin arrangements in nuclei of diploid human cells
A quantitative comparison of higher-order chromatin arrangements was performed in human cell types with three-dimensionally (3D) preserved, differently shaped nuclei. These cell types included flat-ellipsoid n...
