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Article
Open AccessUnderstanding and applying biological resilience, from genes to ecosystems
The natural world is under unprecedented and accelerating pressure. Much work on understanding resilience to local and global environmental change has, so far, focussed on ecosystems. However, understanding a ...
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Article
Open AccessThe giant diploid faba genome unlocks variation in a global protein crop
Increasing the proportion of locally produced plant protein in currently meat-rich diets could substantially reduce greenhouse gas emissions and loss of biodiversity1. However, plant protein production is hampere...
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Article
Genomic regions associated with chocolate spot (Botrytis fabae Sard.) resistance in faba bean (Vicia faba L.)
Chocolate spot (CS), caused by Botrytis fabae Sard., is an important threat to global faba bean production. Growing resistant faba bean cultivars is, therefore, paramount to preventing yield loss. To date, there ...
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Article
VC1 catalyses a key step in the biosynthesis of vicine in faba bean
Faba bean (Vicia faba L.) is a widely adapted and high-yielding legume cultivated for its protein-rich seeds1. However, the seeds accumulate the pyrimidine glucosides vicine and convicine, which can cause haemoly...
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Article
Open AccessChromosome-scale genome assembly provides insights into rye biology, evolution and agronomic potential
Rye (Secale cereale L.) is an exceptionally climate-resilient cereal crop, used extensively to produce improved wheat varieties via introgressive hybridization and possessing the entire repertoire of genes necess...
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Protocol
Transposon-Based Tagging In Silico Using FastPCR Software
Retrotransposons are ubiquitous, generally dispersed components of eukaryotic genomes. These properties, together with their “copy and paste” lifecycle that generates insertional polymorphism without need for ...
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Article
Open AccessHigh-throughput retrotransposon-based genetic diversity of maize germplasm assessment and analysis
Maize is one of the world’s most important crops and a model for grass genome research. Long terminal repeat (LTR) retrotransposons comprise most of the maize genome; their ability to produce new copies makes ...
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Article
Open AccessTRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools
Chromosome-scale genome sequence assemblies underpin pan-genomic studies. Recent genome assembly efforts in the large-genome Triticeae crops wheat and barley have relied on the commercial closed-source assembl...
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Article
Open AccessPalindromic sequence-targeted (PST) PCR: a rapid and efficient method for high-throughput gene characterization and genome walking
Genome walking (GW) refers to the capture and sequencing of unknown regions in a long DNA molecule that are adjacent to a region with a known sequence. A novel PCR-based method, palindromic sequence-targeted P...
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Article
Open AccessAuthor Correction: Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch
In the version of this article initially published, there was a mistake in the calculation of the nucleotide mutation rate per site per generation: 1 × 10−9 mutations per site per generation was used, whereas 9.5...
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Article
Open AccessCloning of the wheat Yr15 resistance gene sheds light on the plant tandem kinase-pseudokinase family
Yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating fungal disease threatening much of global wheat production. Race-specific resistance (R)-genes are used to control rust diseases,...
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Chapter
The Repetitive Landscape of the Barley Genome
While transposable elements (TEs) comprise the bulk of plant genomic DNA, how they contribute to genome structure and organization is still poorly understood. Especially, in large genomes where TEs make the ma...
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Article
Open AccessThe repetitive landscape of the 5100 Mbp barley genome
While transposable elements (TEs) comprise the bulk of plant genomic DNA, how they contribute to genome structure and organization is still poorly understood. Especially in large genomes where TEs make the maj...
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Article
Open AccessGenome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch
Victor Albert, Petri Auvinen, Ykä Helariutta, Jaakko Kangasjärvi and colleagues report the reference genome of the silver birch (Betula pendula) and resequencing of 150 birch individuals. They infer past populati...
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Article
Open AccessConstruction of a map-based reference genome sequence for barley, Hordeum vulgare L.
Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. ‘Morex’ was constructed by the Internati...
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Article
Open AccessA chromosome conformation capture ordered sequence of the barley genome
Cereal grasses of the Triticeae tribe have been the major food source in temperate regions since the dawn of agriculture. Their large genomes are characterized by a high content of repetitive elements and larg...
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Article
Open AccessGene Deletion in Barley Mediated by LTR-retrotransposon BARE
A poly-row branched spike (prbs) barley mutant was obtained from soaking a two-rowed barley inflorescence in a solution of maize genomic DNA. Positional cloning and sequencing demonstrated that the prbs mutant re...
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Article
Retrotransposon molecular markers resolve cocoyam (Xanthosoma sagittifolium) and taro (Colocasia esculenta) by type and variety
Retrotransposon-based molecular markers were applied for the first time within the genera Xanthosoma and Colocasia to assess intraspecific variability among 27 accessions of cocoyam (Xanthosoma sagittifolium) and...
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Article
Development of IRAP- and REMAP-derived SCAR markers for marker-assisted selection of the stripe rust resistance gene Yr15 derived from wild emmer wheat
Yr15 provides broad resistance to stripe rust, an important wheat disease. REMAP- and IRAP-derived co-dominant SCAR markers were developed and localize Yr15 ...
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Article
Evaluation of marker-assisted selection for the stripe rust resistance gene Yr15, introgressed from wild emmer wheat
Stripe rust disease is caused by the fungus Puccinia striiformis f. sp. tritici and severely threatens wheat worldwide, repeatedly breaking resistance conferred by resistance genes and evolving more aggressive st...
