15 Result(s)
within
English
Life Sciences
Biochemistry, general
Sung-** Kim
Protocol
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Protocol
Compact Eight-Channel Light-Sensing System for Bioassays
The present protocol introduces a new instrumental setup as a luminometer to simultaneously measure eight light samples with high sensitivity. The system consists of 8-channel photomultiplier tubes (8-PMTs) wi...
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Protocol
Molecular Tension Probe for In Vitro Bioassays
Cell-free bioassays (CFBs) provide their own distinctive merits over cell-based bioassays (CBBs) including (i) rapid and on-site applicability, (ii) long-term utility, and (iii) bioanalytical versatility. The ...
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Protocol
One-Channel Microsliding Luminometer for Quantifying Low-Energy Bioluminescent Lights
A high-throughput quantitative determination of multiple light-emitting samples is a virtue of many light determination systems. In this chapter, we introduce a compact and efficient light determination system...
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Protocol
Quantitative Imaging of Retinoic Acid Activities in Living Mammalian Cells
Retinoic acid (RA) is an intriguing metabolite that is necessary for embryonic development and differentiation in vertebrates. The present protocol demonstrates how to image RA activities indirectly in mammali...
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Protocol
Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging
The present protocol introduces a visible light bioluminescence imaging (BLI) platform together with 12 novel coelenterazine (CTZ) analogues and luciferase sets. We exemplify to create diverse hues of biolumin...
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Protocol
Methods for the Study of Entotic Cell Death
Entosis is a mechanism of cell competition occurring in cancers that involves the engulfment and killing of neighboring cells. The death of ingested cells, called entotic cell death, usually occurs in a non-ap...
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Protocol
A Bioluminescence Assay System for Imaging Metal Cationic Activities in Urban Aerosols
A bioluminescence-based assay system was fabricated for an efficient determination of the activities of air pollutants. The following four components were integrated into this assay system: (1) an 8-channel as...
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Protocol
A Multichannel Bioluminescence Determination Platform for Bioassays
The present protocol introduces a multichannel bioluminescence determination platform allowing a high sample throughput determination of weak bioluminescence with reduced standard deviations. The platform is d...
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Protocol
Single-Chain Probes for Illuminating Androgenicity of Chemicals
The present protocol introduces a single-chain probe carrying a functional peptide in the N-terminal domain of the androgen receptor (AR NTD) for illuminating androgenicity of ligands. In the single-chain prob...
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Protocol
Circular Permutation Probes for Illuminating Phosphorylation of Estrogen Receptor
The present protocol demonstrates a new strategy for imaging ligand-triggered protein phosphorylation using circularly permutated luciferases (cpLuc): (1) a luciferase is first fragmented into two segments for...
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Protocol
Multicolor Imaging of Bifacial Activities of Estrogens
The present protocol introduces multicolor imaging of bifacial activities of an estrogen. For the multicolor imaging, the authors fabricated two single-chain probes emitting green or red bioluminescence (named...
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Protocol
Fabrication of Molecular Strain Probes for Illuminating Protein–Protein Interactions
A unique bioluminescent imaging probe is introduced for illuminating molecular tension appended by protein–protein interactions (PPIs) of interest. A full-length luciferase is sandwiched between two proteins o...
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Protocol
How to Fabricate Functional Artificial Luciferases for Bioassays
The present protocol introduces fabrication of artificial luciferases (ALuc®) by extracting the consensus amino acids from the alignment of copepod luciferase sequences. The made ALucs have unique sequential iden...
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Protocol
An ALuc-Based Molecular Tension Probe for Sensing Intramolecular Protein–Protein Interactions
Optical imaging of protein–protein interactions (PPIs) facilitates comprehensive elucidation of intracellular molecular events. The present protocol demonstrates an optical measure for visualizing molecular te...
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Protocol
Investigating the Cellular BMK1/ERK5 Signaling Pathway
Big mitogen-activated protein kinase 1 (BMK1), also known as extracellular signal-regulated kinase 5 (ERK5), is the most recently identified member of the mammalian mitogen-activated protein kinase (MAPK) fami...
