3 Result(s)
within
Protocol
Rahul M. Kohli
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Protocol
High-Resolution Analysis of 5-Hydroxymethylcytosine by TET-Assisted Bisulfite Sequencing
DNA cytosine modification is an important epigenetic mechanism that serves critical functions in a variety of biological processes in development and disease. 5-Methylcytosine (5mC) and 5-hydroxymethylcytosine...
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Protocol
Harnessing Alternative Substrates to Probe TET Family Enzymes
TET family enzymes normally oxidize 5-methylcytosine (5mC) in DNA, and play critical roles in sha** the epigenome. Despite their importance, assessing TET activity can be difficult, particularly given the ch...
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Protocol
Bisulfite-Free Sequencing of 5-Hydroxymethylcytosine with APOBEC-Coupled Epigenetic Sequencing (ACE-Seq)
Here, we provide a detailed protocol for our previously published technique, , which localizes 5-hydroxymethylcytosine at single nucleotide resolution using nanogram quantities of input genomic DNA. In addit...
