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  1. No Access

    Article

    Joint single-cell profiling resolves 5mC and 5hmC and reveals their distinct gene regulatory effects

    Oxidative modification of 5-methylcytosine (5mC) by ten-eleven translocation (TET) DNA dioxygenases generates 5-hydroxymethylcytosine (5hmC), the most abundant form of oxidized 5mC. Existing single-cell bisulf...

    Emily B. Fabyanic, Peng Hu, Qi Qiu, Kiara N. Berríos… in Nature Biotechnology (2024)

  2. No Access

    Article

    Direct enzymatic sequencing of 5-methylcytosine at single-base resolution

    5-methylcytosine (5mC) is the most important DNA modification in mammalian genomes. The ideal method for 5mC localization would be both nondestructive of DNA and direct, without requiring inference based on de...

    Tong Wang, Johanna M. Fowler, Laura Liu, Christian E. Loo… in Nature Chemical Biology (2023)

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    Article

    Controllable genome editing with split-engineered base editors

    DNA deaminase enzymes play key roles in immunity and have recently been harnessed for their biotechnological applications. In base editors (BEs), the combination of DNA deaminase mutator activity with CRISPR–C...

    Kiara N. Berríos, Niklaus H. Evitt, Rachel A. DeWeerd, Diqiu Ren… in Nature Chemical Biology (2021)

  4. No Access

    Protocol

    Bisulfite-Free Sequencing of 5-Hydroxymethylcytosine with APOBEC-Coupled Epigenetic Sequencing (ACE-Seq)

    Here, we provide a detailed protocol for our previously published technique, , which localizes 5-hydroxymethylcytosine at single nucleotide resolution using nanogram quantities of input genomic DNA. In addit...

    Tong Wang, Meiqi Luo, Kiara N. Berrios, Emily K. Schutsky, Hao Wu… in DNA Modifications (2021)