59 Result(s)
within
Protocol
Pan **g
Life Sciences
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Protocol
Amino Acid Analysis With Postcolumn Fluorescent Derivatization
Amino acid analysis may be considered to be a fundamental technique for all aspects of protein chemistry. It is used not only for obtaining new data, but also for confirming the results from other types of ana...
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Engineered Properties and Assays for Human DNase I Mutants
Human deoxyribonuclease (DNase I) is an important clinical agent currently used in the treatment of cystic fibrosis (CF) patients (1). It is inhaled into the airways, where it degrades DNA to lower molecular weig...
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Identification of Efficient Cleavage Sites in Long-Target RNAs
In this chapter, we describe a procedure for identification of efficient hammerhead ribozyme (hRz) cleavage sites in target RNAs. An active hRz library, containing randomized recognition sequences flanked by f...
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Isolation, Culture, and Plant Regeneration From Echinacea purpurea Protoplasts
A plant regeneration system from the isolated protoplasts of Echinacea purpurea L. using an alginate solid/liquid culture is described in the chapter. Viable protoplasts were isolated from 100 mg of young leaves ...
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Quantitative Proteomics by Stable Isotope Labeling and Mass Spectrometry
The goal of quantitative proteomics is to systematically study static state or perturbation-induced changes in protein profile. Most of the recently developed mass spectrometry (MS)-based quantitative proteomi...
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Visualization of Syntenic Relationships With SynBrowse
Synteny is the preserved order of genes between related species. To detect syntenic regions one usually first applies sequence comparison methods to the genomic sequences of the considered species. Sequence si...
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Analysis of Genetic Interactions on a Genome-Wide Scale in Budding Yeast: Diploid-Based Synthetic Lethality Analysis by Microarray
Comprehensive collections of open reading frame (ORF) deletion mutant strains exist for the budding yeast Saccharomyces cerevisiae. With great prescience, these strains were designed with short molecular bar code...
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Effects of Sterols on the Development and Aging of Caenorhabditis elegans
Although Caenorhabditis elegans lacks several components of the de novo sterol biosynthetic pathway, it requires sterols as essential nutrients. Supplemental cholesterol undergoes extensive enzymatic modification...
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Proteomic Analysis of the Sterol-Mediated Signaling Pathway in Caenorhabditis elegans
Since Caenorhabditis elegans is incapable of de novo cholesterol biosynthesis, it must utilize other nonpermissive sterols that are present in the environment by converting them into cholesterol for cellular func...
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In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells
Adult bone marrow stem cell is an ideal target for gene therapy of genetic diseases, selected malignant diseases, and AIDS. The in vivo approach of lentivirus vector (LV)-mediated stem cell gene transfer by in...
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Detection of SPO11-Oligonucleotide Complexes from Mouse Testes
The SPO11 protein generates programmed DNA double-strand breaks (DSBs) that initiate meiotic recombination. Endonucleolytic cleavage 3′ to the DSB sites releases SPO11 from DNA, leaving SPO11 covalently associ...
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Proteomic Analysis of Caenorhabditis elegans
Proteomic studies of the free-living nematode Caenorhabditis elegans have recently received great attention because this animal model is a useful platform for the in vivo study of various biological problems rele...
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Primer-Free Aptamer Selection Using a Random DNA Library
Aptamers are high-affinity oligonucleotides which can be selected from large random libraries by systematic evolution of ligands by exponential enrichment (SELEX) protocols, with affinities and specificities c...
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Monitoring MicroRNA Expression During Embryonic Stem-Cell Differentiation Using Quantitative Real-Time PCR (qRT-PCR)
Quantitative real-time PCR (qRT-PCR) is a reliable method to determine and monitor microRNA (miRNA) expression profiles in different cells, tissues, and organisms. Although there are several different strategi...
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Nanofluidic Devices for Rapid Continuous-Flow Bioseparation
Compared with conventional gel-based techniques, such as gel electrophoresis, which are routinely used for bioseparation in biology and biomedical laboratories, nanofluidic devices with regular engineered siev...
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Determining Gene Flow in Transgenic Cotton
Gene flow is one of the major concerns associated with the release of transgenic plants into the environment. Unrestricted gene flow can results in super weeds, reduction in species fitness and genetic diversi...
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Epistasis, Complexity, and Multifactor Dimensionality Reduction
Genome-wide association studies (GWASs) and other high-throughput initiatives have led to an information explosion in human genetics and genetic epidemiology. Conversion of this wealth of new information about...
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Quantitative Glycoproteomics for N-Glycoproteome Profiling
Glycoproteomics, the global analysis of glycoproteins, is a subfield of proteomics with high biological and clinical relevance. Glycoproteomics integrates knowledge and techniques drawing from glycotechnology,...
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Quantitative Metaproteomics: Functional Insights into Microbial Communities
Quantitative metaproteomics aims to accurately determine the relative abundances of thousands of proteins in a microbial community. This approach can be used to provide a comprehensive view of metabolic activi...
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Analysis of Methanotroph Community Structure Using a pmoA-Based Microarray
The analysis of methanotroph community composition is relevant to studies of methane oxidation in a number of environments where methane is a significant carbon source. The development and application of a mic...
