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Quantitative Glycoproteomics for N-Glycoproteome Profiling

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Shotgun Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1156))

Abstract

Glycoproteomics, the global analysis of glycoproteins, is a subfield of proteomics with high biological and clinical relevance. Glycoproteomics integrates knowledge and techniques drawing from glycotechnology, separation science, mass spectrometry, and bioinformatics to support systematic identification and quantification of glycoproteins in a complex biological sample. In this chapter, we describe a shotgun proteomics-based glycoproteomics platform that provides precise map** of protein N-glycosylation sites and quantitative assessment of glycosylation site occupancy on a global scale.

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Acknowledgment

This work was supported in part with federal funds from the National Institutes of Health under grants K25CA137222 and R21CA149772.

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Authors and Affiliations

  1. Department of Medicine, University of Washington, 1959 NE Pacific Street, Seattle, WA, 98195, USA

    Sheng Pan Ph.D.

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  1. Sheng Pan Ph.D.
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Correspondence to Sheng Pan Ph.D. .

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Editors and Affiliations

  1. Ludwig-Maximilians-University, Munich, Germany

    Daniel Martins-de-Souza

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Pan, S. (2014). Quantitative Glycoproteomics for N-Glycoproteome Profiling. In: Martins-de-Souza, D. (eds) Shotgun Proteomics. Methods in Molecular Biology, vol 1156. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0685-7_25

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  • DOI: https://doi.org/10.1007/978-1-4939-0685-7_25

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0684-0

  • Online ISBN: 978-1-4939-0685-7

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