24 Result(s)
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Protocol
Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19
COVID-19 is a global pandemic caused by the highly infectious SARS-CoV-2 virus. Efforts to combat SARS-CoV-2 infection include mass vaccination and development of monoclonal and convalescent plasma therapeutic...
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Protocol
Detection of Microorganisms in Body Fluid Samples
Next-generation sequencing (NGS) has been widely applied to the identification of microbiome in body fluids. The methodology of 16S rRNA amplicon sequencing is simple, fast, and cost-effective. It overcomes th...
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Protocol
Analysis of Protein Degradation in Ferroptosis
The ubiquitin-proteasome system (UPS) is a highly conserved cellular mechanism that degrades and recycles proteins in eukaryotic cells. It involves the tagging of specific target proteins with ubiquitin, a sma...
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Protocol
Cell-Free RNA Sequencing from Biofluid Samples
Liquid biopsy has obvious advantages over invasive sampling methods. Cell-free DNA is now widely used in prenatal and cancer diagnosis. Cell-free RNA is relatively unstable but could contain potential biomarke...
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Protocol
Confocal Scanning Laser Ophthalmoscopy to Image Retinal Ganglion Cells in Real-Time
Real-time imaging of retinal ganglion cells (RGCs) provides an opportunity for detailed investigation of retinal development, disease mechanisms, and the evaluation of interventions affecting ocular structures...
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Protocol
Exosomal RNA Sequencing from Body Fluid Samples
Exosomes are microcapsules released by different types of cells. It contains DNA, RNA, proteins, and other substances. Exosomes spread in body fluids, and their contents can be phagocytosed by other cells and ...
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Protocol
Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach
Overlap extension PCR is one of the routinely used methods to generate mutagenic genes for the functional and structural study of proteins. However, it is time-consuming to design the overlap** mutagenic pri...
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Protocol
Preparation of Trimethyl Chitosan-Based Polyelectrolyte Complexes for Peptide Subunit Vaccine Delivery
A variety of delivery vehicles have been explored as adjuvanting/delivery platforms for peptide-based subunit vaccines. Polysaccharide-based systems have been found to be especially attractive due to their imm...
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Protocol
Methods for Studying Mouse and Human Invariant Natural Killer T Cells
Invariant natural killer T (iNKT) cells are a unique subset of T lymphocytes that recognize lipid antigens presented by nonpolymorphic major histocompatibility complex (MHC) I-like molecule CD1d. iNKT cells pl...
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Protocol
Assessing Cytochrome P450 Time-Dependent Inhibition (IC50 Shift Assay) Using Both Diluted and Non-Diluted Methods
Evaluation of the time-dependent inhibition (TDI) of cytochrome P450 (CYP) enzymes is very important for understanding the drug–drug interactions. TDI is usually studied by measuring the half-maximal inhibitor...
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Protocol
High-Throughput Measurement for Toxic Effects of Metal Mixtures in Caenorhabditis elegans
The contamination of heavy metals, a class of naturally occurring and persistent toxicants, has become a major public health concern due to increasing industrial and anthropogenic activities. The use of COPAS ...
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Protocol
Nanopore Sequencing and Its Clinical Applications
Nanopore sequencing is a method for determining the order and modifications of DNA/RNA nucleotides by detecting the electric current variations when DNA/RNA oligonucleotides pass through the nanometer-sized ho...
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Protocol
Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution
Activation-induced cytidine deaminase (AID) initiates somatic hypermutation (SHM) by converting deoxycytidines (dC) to deoxyuracils (dU) which then can induce other mutations, and plays a central role in intro...
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Protocol
Surface Plasmon Resonance Spectroscopy for Detection of S-Nitrosylated Proteins
Protein S-nitrosylation, the NO-dependent regulatory mechanism, is a posttranslational modification of reactive cysteine thiols to form S-nitrosothiols. The biotin-switch technique (BST) has become a mainstay met...
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Protocol
Quantitative Fluorescence Measurements with Multicolor Flow Cytometry
Multicolor flow cytometer assays are routinely used in clinical laboratories for immunophenoty**, monitoring disease and treatment, and determining prognostic factors. However, existing methods for quantitat...
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Protocol
Assembly and Assessment of DNA Scaffolded Vaccines
Vaccines play an important role in preventing many life-threatening infectious diseases. To meet the demand of vaccination for treating a wide range of diseases, rational vaccine design has been recognized as ...
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Protocol
Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase
Crop improvement is a never ending process. With a transgenesis approach, it is not inconceivable to envision a continuous addition of new transgenes to existing cultivars. Previously, we described a recombina...
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Protocol
Use of E. coli for the Production of a Single Protein
E. coli has been widely used for recombinant protein production. Here, we introduce a novel expression method in E. coli, the Single Protein Production (SPP) system, in which E. coli is converted i...
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Protocol
Analysis of Changes in Protein Level and Subcellular Localization During Cell Cycle Progression Using the Budding Yeast Saccharomyces cerevisiae
Methods are described here to monitor changes in protein level and subcellular localization during the cell cycle progression in the budding yeast Saccharomyces cerevisiae. Cell synchronization is achieved by an ...
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Protocol
Atomic Force Microscopy Studies on Circular DNA Structural Changes by Vincristine and Aspirin
In this chapter, we have presented materials and methods to study the interaction between DNA and small molecule drugs by AFM. The detailed AFM imaging of the circular DNA after incubation with various concen...
