24 Result(s)
within
Protocol
Li**g Du
Biomedicine
English
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Protocol
A Nonviral piggyBac Transposon-Mediated Method to Generate Large-Scale CAR-NK Cells from Human Peripheral Blood Primary NK Cells
With the inherent antitumor function and unique “off-the-shelf” potential, genetically engineered human natural killer (NK) cells with chimeric antigen receptors (CARs) bear great promise for the treatment of ...
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Metabolite Extraction from RPE Cells and Retinas Related to Retinitis Pigmentosa
The application of metabolomics in ophthalmology helps to identify new biomarkers and elucidate disease mechanisms in different eye diseases, as well as aiding in the development of potential treatment options...
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Measuring Autophagosome Flux
Autophagy is an evolutionarily conserved catabolic process that plays an import role in cellular proteostasis. The continual degradation, and recycling, of portions of the cytoplasm through autophagy eliminate...
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Quantification of Receptor Association, Dissociation, and NA-Dependent Motility of Influenza A Particles by Biolayer Interferometry
We describe a method for real-time analysis and quantification of influenza A virus (IAV)–receptor interactions by biolayer interferometry (BLI). Biotinylated synthetic sialoglycans or sialoglycoproteins (biot...
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Guidance for SARS-CoV-2 RNA-Based Molecular Assay Analytical Performance Evaluations
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is frequently diagnosed through detection of viral RNA using nucleic acid amplification testing (NAAT) assays that are usually used in cen...
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Studying Mitochondrial Network Formation by In Vivo and In Vitro Reconstitution Assay
Mitochondria change their morphologies from small isolated vesicles to large continuous networks across the cell cycles. The mitochondrial network formation (MNF) plays an important role in maintaining mitocho...
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Diagnosis and Treatment of Breast Cancer in the Precision Medicine Era
Breast cancer is one of the most leading causes of death for women worldwide. According to statistics published by the International Agency for Research on Cancer (IARC), the incidence of breast cancer is on t...
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Analysis of the Functional States of an Astrocyte Syncytium
In the central nervous system (CNS), astrocytes are cytoplasmically interconnected through specialized conduit proteins, called gap junctions. This establishes a syncytial network that runs across the entire b...
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Orthotopic Pancreatic Tumor Mouse Models of Liver Metastasis
The survival from pancreatic cancer is poor because most patients are diagnosed after the cancer has metastasized. Liver is the most common site of pancreatic cancer metastasis. Orthotopic mouse models of live...
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In Silico Strategies to Design Small Molecules to Study Beta-Amyloid Aggregation
The amyloid cascade hypothesis of Alzheimer’s disease is an intriguing and complex pathway which alludes to the accumulation of amyloid (Aβ) aggregates as a key toxic event. In this regard, computational tools...
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Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis
The cell envelope of Gram-negative bacteria comprises an outer membrane, a cytoplasmic inner membrane, and an interstitial space. The tripartite multidrug transporter AcrAB-TolC, which uses proton electrochemi...
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Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies
The activation of ATM is critical in the DNA double strand breaks repair pathway. Acetylation of ATM by Tip60 histone acetyltransferase (HAT) plays a key role in the activation of ATM kinase activity in respon...
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Monitoring and Modulation of Inducible Foxp3+ Regulatory T-Cell Differentiation in the Lymph Nodes Draining the Small Intestine and Colon
The mucosa-draining lymphoid tissue favors differentiation of inducible Foxp3+ regulatory T cells. Adoptive transfer of T-cell receptor (TCR) transgenic (Tg) T cells is a powerful tool to study antigen-specific r...
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Reverse Genetics for Porcine Reproductive and Respiratory Syndrome Virus
Porcine reproductive and respiratory syndrome (PRRS) is a reemerging swine disease, and has become economically the most significant disease in pork production worldwide. The causative agent is PRRS virus (PRR...
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In Silico Prediction of RNA Secondary Structure
The secondary structure of an RNA molecule represents the base-pairing interactions within the molecule and fundamentally determines its overall structure. In this chapter, we overview the main approaches and ...
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Monitoring Phosphatidic Acid Signaling in Breast Cancer Cells Using Genetically Encoded Biosensors
Phospholipids are important signaling molecules that regulate cell proliferation, death, migration, and metabolism. Many phospholipid signaling cascades are altered in breast cancer. To understand the function...
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Analysis of Invadopodia Formation in Breast Cancer Cells
Metastasis is the major cause of breast cancer deaths. To spread from the primary tumor sites to distant tissues, solid tumor cells need to degrade the surrounding extracellular matrix (ECM). The protrusive me...
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Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System
Long-term hematopoietic stem cells (LT-HSCs) have the ability to self-renew and differentiate into all blood cell lineages. Understanding the genetic networks that regulate LT-HSC function in the adult bone ma...
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Purification, Quantification, and Functional Analysis of Complement Factor H
Complement Factor H (FH) is an abundant, non-enzymic plasma/serum glycoprotein, which has a major role in regulating activation of the complement system. It can be purified from human plasma/serum by affinity ...
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miRNA and shRNA Expression Vectors Based on mRNA and miRNA Processing
RNA interference (RNAi) has emerged as a powerful tool in basic research and therapeutics by silencing the expression of specific target genes. RNAi is triggered by double-stranded small interfering RNAs, whic...
