5 Result(s)
within
Protocol
Johannes C. Walter
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Protocol
Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation
Faithful chromosome segregation during cell division requires proper bipolar spindle assembly and critically depends on spindle pole integrity. In most animal cells, spindle poles form as the result of the con...
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Protocol
Replication-Coupled DNA Interstrand Cross-Link Repair in Xenopus Egg Extracts
Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA ...
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Protocol
Construction of Plasmids Containing Site-Specific DNA Interstrand Cross-Links for Biochemical and Cell Biological Studies
Plasmids containing a site-specific DNA interstrand cross-link (ICL) are invaluable tools for the investigation of ICL repair pathways at the biochemical and cellular level. We describe a procedure for prepara...
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Protocol
DNA Replication in Nucleus-Free Xenopus Egg Extracts
Extracts derived from Xenopus laevis eggs represent a powerful cell-free system to study eukaryotic DNA replication. A variation of the system allows for DNA replication not only in a cell-free environment, but a...
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Protocol
Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs
Cytoplasmic egg extracts from the frog Xenopus laevis represent a powerful cell-free system to study eukaryotic chromosomal DNA replication. In the classical approach, sperm chromatin is added to unfractionated e...
