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    Protocol

    Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg Extracts

    Xenopus egg extracts provide a cell-free system to analyze various aspects of chromatin biology. Here we describe a modified method of chromatin immunoprecipitation (ChIP) to detect the interaction of proteins wi...

    Kelly B. Wolfe, David T. Long in DNA Repair (2019)

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    Protocol

    Construction of Plasmids Containing Site-Specific DNA Interstrand Cross-Links for Biochemical and Cell Biological Studies

    Plasmids containing a site-specific DNA interstrand cross-link (ICL) are invaluable tools for the investigation of ICL repair pathways at the biochemical and cellular level. We describe a procedure for prepara...

    Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter… in DNA Repair Protocols (2012)