5 Result(s)
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Protocol
In Vivo Evaluation of Dystrophin Exon Skip** in mdx Mice
Dystrophin exon skip** in mdx mice has been the key model for the development of antisense therapy in vivo. Evaluation of exon skip** in this model involves the following two aspects: (1) efficiency and accur...
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Protocol
Biochemical Identification of Nonmethylated DNA by BioCAP-Seq
CpG islands are regions of vertebrate genomes that often function as gene regulatory elements and are associated with most gene promoters. CpG island elements usually contain nonmethylated CpG dinucleotides, w...
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Protocol
Systemic Delivery of Antisense Oligomer in Animal Models and Its Implications for Treating DMD
Antisense oligomer (AO)-mediated splicing modulation for treating DMD demands a systemic administration of AOs as pharmacological drugs to achieve effective prevention of disease progression and to improve qua...
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Protocol
Construction of Plasmids Containing Site-Specific DNA Interstrand Cross-Links for Biochemical and Cell Biological Studies
Plasmids containing a site-specific DNA interstrand cross-link (ICL) are invaluable tools for the investigation of ICL repair pathways at the biochemical and cellular level. We describe a procedure for prepara...
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Protocol
Monitoring the Temporal and Spatial Distribution of RNA in Living Yeast Cells
RNA localization is a cellular process to spatially restrict translation of specific proteins to defined regions within or between cells. Most localized mRNAs contain cis-acting localization elements in the 3′-un...
