17 Result(s)
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Chapter
S.S. Deshpande
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Antibodies: Biochemistry, Structure, and Function
An understanding of the basic principles of the biology of the vertebrate immune system and its regulation is a prerequisite for the successful experimental production of antibodies. Such knowledge helps in av...
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Conjugation Techniques
Preparation of well-defined conjugates during various stages, from hapten immunogen preparation for antibody production to that of tracer or labeling reagents such as enzyme-antigen or enzyme-antibody conjugat...
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Enzymes and Signal Amplification Systems
Since the introduction of radioimmunoassays in the late 1950s and the widespread reliance on radioisotopic labels in various fields of clinical and medical science and practice during the 1960s and 1970s, rapi...
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Immunoassay Classification and Commercial Technologies
Immunoassays encompass techniques for the detection and quantification of antigens or antibodies, and are one of the most powerful of all immunochemical techniques. Although the term “immunoassay” generally re...
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Data Analysis
Data analysis is an important aspect of using solid-phase immunoassays. The basic principles of obtaining dose-response curves using immunoassay techniques were described earlier in this book. In quantitative ...
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Introduction
Qualitative and quantitative analytical techniques are of tremendous importance in all fields and disciplines of life sciences for the detection, identification, and measurement of concentration of a wide vari...
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Antigen-Antibody Reactions
Enzyme immunoassays are based on antigen-antibody reactions involving enzyme-labeled antigen (or antibody) with antibody (or antigen). An understanding of the fundamentals of antigen-antibody reactions is, the...
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Antibody Production
The production and large scale availability of antibody of desired affinity and specificity and/or cross-reactivity are fundamental to the process of develo** sensitive, precise, and specific immunoassay pro...
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Separation and Solid-Phase Systems
To quantitate the amount of analyte present in an enzyme immunoassay (EIA), the extent of reaction of the enzyme-labeled ligand (or antibody) with antibody (or the ligand) must be determined. Since the reactio...
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Assay Development, Evaluation, and Validation
Modern immunoassay systems exist in several different formats with diverse ancillary technologies. An ideal immunoassay for a given need depends upon the kind of information desired and the physical environmen...
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Reagent Formulations and Shelf Life Evaluation
The control of assay quality and the degree of quality control required begin with the source and the accurate preparation of reagents and control sera used in the assay procedure. The quality of reagents used...
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Documentation, Registration, and Diagnostic Start-Ups
Commercial production of immunodiagnostic kits requires that the various components be manufactured consistently over a long period of time. The process involves availability of a consistent source of antibody...
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Improving nutritional quality of cool season food legumes
Nutritional quality of food legumes includes the composition and functionality of the seeds. Composition is the main factor affecting nutritional value (contributions to energy and protein requirements, amino ...
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Introduction
Never in the history of humankind did we face such monumental challenges and daunting prospects as we do approaching the dawn of the twenty-first century. The present world population of about 5 billion and it...
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Legumes
Legumes (including soybeans and peanuts) are ranked fifth in terms of annual world grain production, at around 170 million metric tons, after wheat, rice, corn, and barley (Table 2-1; cf. Table 0-1). With the ...
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Cereals
Cereal grains are the major food of humankind. In many of the less developed countries of the world, cereals provide two-thirds or more of the dietary calories (Deshpande and Damodaran 1990). Rice in Asia, cor...
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Chemistry and Safety of Plant Polyphenols
Phenolic compounds are widely distributed in plant kingdom and are considered to be secondary metabolites. They do not seem to be essential for plant life, at least at the cellular level. Plants provide nearly...
