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Chapter
Transgenic Ryegrasses(Loliumspp.)
Ryegrasses (Lolium spp.), together with the closely related fescues (Festucaspp.), are of significant value in temperate grasslands (Jauhar 1993). The commercially most important ryegrasses are Italian or annual ...
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Chapter
Establishment of and Plant Regeneration from Embryogenic Cell Suspensions and their Protoplasts in Forage Grasses
An efficient system for green plant regeneration from embryogenic suspensions and corresponding protoplasts in the temperate forage and turf grass species: Festuca arundinacea, F. pratensis, F. rubra, Lolium mult...
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Chapter
Polyethylene Glycol-Mediated Direct Gene Transfer to Tobacco Protoplasts and Regeneration of Transgenic Plants
The protocol detailed below describes the procedure for the transient and stable transformation of leaf protoplasts obtained from axenic shoot cultures of Nicotiana tabacum L. cv. Petit Havana SR1, a widely used ...
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Chapter
Genetic Transformation in Festuca arundinacea Schreb. (Tall Fescue) and Festuca pratensis Huds. (Meadow Fescue)
Tall fescue (Festuca arundinacea Schreb.) is a wind-pollinated, highly self-infertile polyploid perennial cool-season forage, turf, and conservation grass. It is indigenous to Europe, also naturally occuring on t...
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Chapter
Standard Molecular Techniques for the Analysis of Transgenic Plants
In the following sections, a series of step-by-step protocols for standard molecular techniques widely used in the characterization of transgenic plants and in gene expression studies with plant cells are comp...
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Chapter
Somatic Hybridization in Festuca and Lolium
Several species in the genus Festuca are commonly grown perennial forage and turf grasses of the temperate geographic region. Particularly tall fescue (F. arundinacea Schreb.) has a wide range of distribution as ...
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Chapter
Somatic Hybridization by Microfusion of Protoplasts
Somatic hybridization in plants has classically relied on en masse fusion of protoplasts. In general, large populations of protoplasts isolated from both parental forms are mixed and subjected to fusogenic tre...