668 Result(s)
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Article
Cellulase production from spent sulfite liquor and paper-mill waste fiber
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Article
The principle of parabolic feed for a fed-batch culture of baker’s yeast
A parabolic nutrient supplementation has been derived by the specific growth rate, sugar conversion rate, and Pasteur effect, followed by high qualitative and quantitative biomass production with the lowest su...
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Article
The microbial production of polyhydroxybutyrate from methanol
One hundred strains of methanol-utilizing bacteria were isolated. From them a methylotrophic strain 8502-3 was selected out for biodegradable plastics polyhydroxybutyrate (PHB) production on methanol. It was n...
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Article
Production and properties of 3-cyanopyridine hydratase inRhodococcus equi SHB-121
A strain ofRhodococcus equi SHB-121 forming 3-cyanopyridine hydratase was screened from nitrile-polluted soil. The optimum conditions for the formation of 3-cyanopyridine hydratase by the strain SHB-121 have been...
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Article
Production, characterization, and application of the cellulase-free xylanase fromAspergillus niger
The effects of carbon source on xylanase and cellulase production were studied. The extract of steam-exploded corn stover was found to be the best raw material for producing cellulase-free xylanase. The xylano...
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Article
Estimation of the optimal concentrations of residual sugar and cell growth rate for a fed-batch culture ofSaccharomyces cerevisiae
Estimation of the optimal concentrations of residual sugar in medium for a fed-batch culture of Baker’s yeast has been studied and practiced. The concentrations, however, depended on different species and targ...
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Article
The production and properties of a new xylose reductase from fungus
Neurospora crassa XI was found to ferment xylose and glucose simultaneously. Xylose was the appropriate inducer for the production of xylose reductase that had two isoenzymes designated as EI and...
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Article
Removal of known, abundant cDNA species by specific double-stranded cDNA synthesis-based subtraction
An efficient and simple method for removing known, abundant cDNA species from the cDNA pool of highly differentiated cells is reported. The method involves preparation of sscDNA, followed by dscDNA-synthesis o...
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Article
Molecular analysis and heterologous expression of the gene encoding methylmalonyl—coenzyme a mutase from rifamycin SV-producing strain Amycolatopsis mediterranei U32
The conversion of succinyl-coenzyme A (CoA) into methylmalonyl-CoA, catalyzed by adenosylcobalamin-dependent methylmalonyl-CoA mutase (MCM), represents an important source of building blocks for rifamycin SV b...
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Article
A novel magnetite-immobilized cell process for heavy metal removal from industrial effluent
The sorption and desorption of copper (II) (Cu[II]) ions from the wastewater by magnetite-immobilized cells of Pseudomonas putida 5-x with acidic pretreatment were studied. Pretreating cells with 0.6 N HCl was fo...
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Article
Mannosylerythritol lipid increases levels of galactoceramide in and neurite outgrowth from PC12 pheochromocytoma cells
We report here that a microbial extracellular glycolipid,mannosylerythritol lipid (MEL), induces the outgrowth ofneurites from and enhances the activity of acetylcholinesterase(AChE) in PC12 pheochromocytoma c...
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Article
Treatment of mouse melanoma cells with phorbol 12-myristate 13-acetate counteracts mannosylerythritol lipid-induced growth arrest and apoptosis
Mannosylerythritol lipid (MEL), an extracellularglycolipid from yeast, induces the differentiation ofHL-60 promyelocytic leukemia cells towardsgranulocytes. We show here that MEL is also a potentinhibitor of t...
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Article
Induction of the differentiation of human HL-60 promyelocytic leukemia cell line by succinoyl trehalose lipids
Four analogs of succinoyl trehalose lipid-3 (STL-3)with saturated even-number or odd-number carbonchains, and unsaturated or halogenated fatty acidswere examined for their ability to inhibit the growthand indu...
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Article
Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from cottonseed oil and valeric acid in batch culture of Ralstonia sp. strain JC-64
A Ralstonia sp. strain JC-64 that is capable of accumulating poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P[3HB-co-3HV]) from cottonseed oil and valeric acid was isolated. By using a high limiting-nitrogen (HLN)...
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Article
Hot topics in adeno-associated virus as a gene transfer vector
Adeno-associated virus (AAV) is a promising viral vector in treating many kinds of hereditary diseases. The broad host range, low level of immune response, and longevity of gene expression observed with this v...
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Article
Rapid expression of recombinant proteins in modified CHO cells using the baculovirus system
Baculovirus containing the mammalianCMV promoter, in place of the insect polyhedronpromoter (BacMam), has been used to transientlytransfect COS, CHO and CHOE1a (CHO cells expressing theE1a transcriptional acti...
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Article
l-Asparaginase release from Escherichia coli cells with aqueous two-phase micellar systems
A method was proposed to release and separate l-asparaginase (EC 3.5.1.1) from Escherichia coli ATCC 11303 cells with aqueous two-phase micellar systems. The systems were composed of K2HPO4 and Triton X-100. The ...
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Article
Alkaline peroxide mechanical pul** of wheat straw with enzyme treatment
Alkaline peroxide mechanical pul** (APMP) of wheat straw with enzyme treatment was studied. Instead of direct enzyme pretreatment on wheat straw, an alternative treatment method was used, in which coarse pul...
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Article
Knockdown of mouse adult β-globin gene expression in MEL cells by retrovirus vector-mediated RNA interference
RNA interference (RNAi) efficiently induces sequence-specific gene silencing in mammalian cells through short interfering RNA (siRNA) of 21–23 nucleotides synthesized in vitro or expressed by DNA-based vector....
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Article
Evaluation of hybridization conditions for spotted oligonucleotide-based DNA microarrays
We compared different hybridization conditions of oligonucleotide-based DNA microarray to acquire optimized and reliable microarray data. Several parameters were evaluated at different hybridization conditions...