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Article
Development of a tetra-primer ARMS PCR-based assay for detection of a novel single-nucleotide polymorphism in the 5′ untranslated region of the bovine ITGB6 receptor gene associated with foot-and-mouth disease susceptibility in cattle
ITGB6 is known to be one of the major receptor components involved in host tropism of foot-and-mouth disease (FMD) virus in cattle. A competitive PCR technique called ARMS PCR was adapted to identify a single-...
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Article
Profiling of Bovine Breast Cancer 1, Early Onset (BRCA1) Gene Among Frieswal (HF × Sahiwal) Cows and Their Association with Mastitis
The main theme of the present study was to investigate whether the bovine breast cancer 1 (BRCA1) gene was associated with mastitis resistance in Frieswal (HF × Sahiwal) cattle. Two SNPs (G22231T, T25025A) wer...
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Article
Heterozygosity at the SNP (rs136500299) of ITGB6 receptor gene possibly influences the susceptibility among crossbred bull to foot and mouth disease infection
Zebu (Bos indicus) cattle are known to be resistant against foot and mouth disease virus (FMDV) compared to taurine (Bos taurus). To understand the susceptibility of two cattle species to FMDV infection in terms ...
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Article
Understanding the mechanisms of ATPase beta family genes for cellular thermotolerance in crossbred bulls
Na+/K+-ATPase is an integral membrane protein composed of a large catalytic subunit (alpha), a smaller glycoprotein subunit (beta), and gamma subunit. The beta subunit is essential for ion recognition as well ...
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Article
SNPs at exonic region of aquaporin-7 (AQP7) gene may affect semen quality parameters among crossbred bulls
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Article
Novel identification of Factor XI deficiency in Indian Sahiwal (Bos indicus) cattle
Factor-XI deficiency (FXID) is inherited as autosomal lethal recessive disorder of carrier Holstein–Friesian bulls. A 76 base pair segment insertion into exon 12 in Factor-XI gene causes FXID in cattle. Keepin...
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Article
Open AccessDevelopment of a fast and economical genoty** protocol for bovine leukocyte adhesion deficiency (BLAD) in cattle
Fast and economical means of assaying SNP’s are important in diagnostic assays, especially when a large number of animals have to be screened for a genetic disease. This study was aimed at the development of a...
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Article
Cytosine deletion at AP2-box region of HSP70 promoter and its influence on semen quality traits in crossbred bulls
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Article
Application of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Cow Components Adulterated in Buffalo Milk/Meat
Loop-mediated isothermal amplification (LAMP) is a diagnostic method for amplification of DNA with rapid and minimal equipment requirement. In the present study, we applied the LAMP assay for rapid detection o...
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Article
Comparative expression profile of NOD1/2 and certain acute inflammatory cytokines in thermal-stressed cell culture model of native and crossbred cattle
Thermotolerance depends mainly on the health and immune status of the animals. The variation in the immune status of the animals may alter the level of tolerance of animals exposed to heat or cold stress. The ...
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Article
RT-LAMP assay: an alternative approach for profiling of bovine heat shock protein 70 gene in PBMC cultured model
The purpose of this study is to develop a novel Reverse Transcriptase Loop-mediated isothermal amplification (RT-LAMP) based assay for in vitro profiling of heat shock protein 70 (Hsp70) in bovine peripheral bloo...
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Article
Differential expression of microRNAs associated with thermal stress in Frieswal (Bos taurus x Bos indicus) crossbred dairy cattle
Environmental temperature is one of the important abiotic factors that influence the normal physiological function and productive performance of dairy cattle. Temperature stress evokes complex responses that a...
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Article
Open AccessT-ARMS PCR genoty** of SNP rs445709131 using thermostable strand displacement polymerase
In a recent publication, we reported the successful use of tetra primer-amplification refractory mutation system based polymerase chain reaction (T-ARMS-PCR) for genoty** of rs445709131-SNP responsible for t...
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Article
Identification of differentially expressed microRNAs in Sahiwal (Bos indicus) breed of cattle during thermal stress
microRNAs (miRNAs) are a class of small non-coding RNAs that play key roles in post transcriptional gene regulation that influence various fundamental cellular processes, including the cellular responses durin...
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Article
Characterization of a putative ribosome binding site at the 5′ untranslated region of bovine heat shock protein 90
Untranslated regions (UTRs) of the transcripts play significant roles in translation regulation and continue to raise many intriguing questions in our understanding of cellular stress physiology. Internal ribo...
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Article
Comparative miRNA signatures among Sahiwal and Frieswal cattle breeds during summer stress
MicroRNAs (miRNAs) are known to take part in different biological mechanisms, including biotic as well as abiotic cellular stresses. The present investigation was aimed to identify comparative expression profi...
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Article
Development of CD163 receptor-based enzyme-linked immunosorbent assay for diagnosis of porcine reproductive and respiratory syndrome virus
Porcine reproductive and respiratory syndrome (PRRS) is an important economical disease in the global swine industry. The accurate detection of the PRRS virus (PRRSV) antigen is essential for the disease contr...
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Article
Profiling of key heat shock proteins and their relationship with male sexual behavior and seminal characteristics in Kankrej (Bos indicus) breeding bulls during different seasons
The goal of this study is to use indirect ELISA to determine the concentration of major heat shock proteins (Hsps) in Kankrej (Bos indicus) breeding bulls and their relationship with certain male phenotypic trait...
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Article
Development of an on-site lateral flow immune assay based on mango leaf derived colloidal silver nanoparticles for rapid detection of Staphylococcus aureus in milk
In order to ensure food safety, screening food samples for the presence of pathogens has been categorised as a legal testing item throughout the globe. One of the most prevalent zoonotic bacteria transmitted t...
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Article
Development of B cell epitopes-based enzyme linked immune sorbent assay for detection of bovine anti-Mullerian hormone
The present study aimed to generate antibodies against predicted B cell epitopic peptides encoding bAMH for develo** different ELISA models. Sandwich ELISA was determined to be an excellent technique for ass...
